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Development and evaluation of a molecular assay for detection of nontuberculous mycobacteria on the basis of the COBAS Amplicor platform


Peter-Getzlaff, S; Lüthy, J; Böddinghaus, B; Böttger, E C; Springer, B (2008). Development and evaluation of a molecular assay for detection of nontuberculous mycobacteria on the basis of the COBAS Amplicor platform. Journal of Clinical Microbiology, 46(12):4023-4028.

Abstract

We have developed and evaluated a semiautomated assay for detection of nontuberculous mycobacteria (NTM) from clinical samples based on the COBAS Amplicor M. tuberculosis test (Roche Diagnostics, Switzerland). A capture probe, specific for mycobacteria at the genus level, was linked to magnetic beads and used for the detection of amplification products obtained by the COBAS Amplicor M. tuberculosis assay. We demonstrate that the analytical sensitivity of the genus assay is similar to that of COBAS Amplicor M. tuberculosis detection. 416 clinical specimens were evaluated for the presence of NTM DNA. Sensitivity for smear positive and smear negative specimens was found to be 100 % and 47.9 %, respectively. Specificity was 97.7 %, the positive predictive value (PPV) 84.6 % and the negative predictive value (NPV) 93.1 %. The genus assay is easy to perform, produces reliable results and was found to be a valuable diagnostic tool for rapid diagnosis of infections with NTM. The genus assay has the potential to detect NTMs not routinely recovered by culture and to discover new mycobacterial species.

We have developed and evaluated a semiautomated assay for detection of nontuberculous mycobacteria (NTM) from clinical samples based on the COBAS Amplicor M. tuberculosis test (Roche Diagnostics, Switzerland). A capture probe, specific for mycobacteria at the genus level, was linked to magnetic beads and used for the detection of amplification products obtained by the COBAS Amplicor M. tuberculosis assay. We demonstrate that the analytical sensitivity of the genus assay is similar to that of COBAS Amplicor M. tuberculosis detection. 416 clinical specimens were evaluated for the presence of NTM DNA. Sensitivity for smear positive and smear negative specimens was found to be 100 % and 47.9 %, respectively. Specificity was 97.7 %, the positive predictive value (PPV) 84.6 % and the negative predictive value (NPV) 93.1 %. The genus assay is easy to perform, produces reliable results and was found to be a valuable diagnostic tool for rapid diagnosis of infections with NTM. The genus assay has the potential to detect NTMs not routinely recovered by culture and to discover new mycobacterial species.

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8 citations in Web of Science®
9 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Microbiology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:22 October 2008
Deposited On:05 Nov 2008 12:58
Last Modified:05 Apr 2016 12:32
Publisher:American Society for Microbiology
ISSN:0095-1137
Additional Information:Copyright: American Society for Microbiology
Publisher DOI:10.1128/JCM.01101-08
PubMed ID:18945835
Permanent URL: http://doi.org/10.5167/uzh-4913

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