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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-49223

Novacco, M; Boretti, F S; Wolf-Jäckel, G A; Riond, B; Meli, M L; Willi, B; Lutz, H; Hofmann-Lehmann, R (2011). Chronic "Candidatus Mycoplasma turicensis" infection. Veterinary Research, 42(1):59.

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Abstract

ABSTRACT: "Candidatus Mycoplasma turicensis" infects felids. The pathogenesis of "Candidatus M. turicensis" chronic infection is poorly understood. The goals of the present study were to (1) induce reactivation of the infection in chronic carrier cats by attempted immunosuppression, (2) identify potential tissue sequestration using real-time TaqMan® PCR and (3) monitor the humoral immune response by DnaK enzyme-linked immunosorbent assay (ELISA). Ten specified pathogen-free cats that had ostensibly recovered from experimental "Candidatus M. turicensis" infection were used: five cats (group 1) received high dose methylprednisolone (attempted immunosuppression), while five cats served as untreated controls (group 2). Besides weekly blood samples, tissue samples were collected from bone marrow, kidney, liver and salivary glands at selected time points. The cats in group 1 had significantly lower lymphocyte counts and higher blood glucose levels after methylprednisolone administration than the controls. After methylprednisolone administration one blood and three tissue samples from cats in group 1 tested PCR-positive; before the administration, only one sample was positive. All other samples tested PCR-negative. All cats stayed seropositive; the antibody levels of the cats in group 1 showed a significant transient decrease after methylprednisolone administration. This is the first study to report the presence of "Candidatus M. turicensis" in tissues of chronically infected cats and the persistence of anti-feline hemoplasma antibodies in the absence of detectable bacteremia. Methylprednisolone administration did not lead to a significant reactivation of the infection. Our results enhance the knowledge of "Candidatus M. turicensis" infection pathogenesis and are clinically relevant to the prognosis of hemoplasma-infected cats.

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinary Clinic > Department of Farm Animals > Clinical Laboratory
05 Vetsuisse Faculty > Veterinary Clinic > Department of Small Animals > Clinic for Small Animal Internal Medicine
DDC:570 Life sciences; biology
630 Agriculture
Language:English
Date:2011
Deposited On:25 Aug 2011 06:53
Last Modified:27 Nov 2013 17:17
Publisher:BioMed Central
ISSN:0928-4249
Publisher DOI:10.1186/1297-9716-42-59
PubMed ID:21507220
Citations:Web of Science®. Times Cited: 7
Google Scholar™
Scopus®. Citation Count: 7

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