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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-49277

Harterink, M; Port, F; Lorenowicz, M J; McGough, I J; Silhankova, M; Betist, M C; van Weering, J R T; van Heesbeen, R G H P; Middelkoop, T C; Basler, K; Cullen, P J; Korswagen, H C (2011). A SNX3-dependent retromer pathway mediates retrograde transport of the Wnt sorting receptor Wntless and is required for Wnt secretion. Nature Cell Biology, 13(8):914-923.

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Wnt proteins are lipid-modified glycoproteins that play a central role in development, adult tissue homeostasis and disease. Secretion of Wnt proteins is mediated by the Wnt-binding protein Wntless (Wls), which transports Wnt from the Golgi network to the cell surface for release. It has recently been shown that recycling of Wls through a retromer-dependent endosome-to-Golgi trafficking pathway is required for efficient Wnt secretion, but the mechanism of this retrograde transport pathway is poorly understood. Here, we report that Wls recycling is mediated through a retromer pathway that is independent of the retromer sorting nexins SNX1-SNX2 and SNX5-SNX6. We have found that the unrelated sorting nexin, SNX3, has an evolutionarily conserved function in Wls recycling and Wnt secretion and show that SNX3 interacts directly with the cargo-selective subcomplex of the retromer to sort Wls into a morphologically distinct retrieval pathway. These results demonstrate that SNX3 is part of an alternative retromer pathway that functionally separates the retrograde transport of Wls from other retromer cargo.

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
DDC:570 Life sciences; biology
Deposited On:02 Sep 2011 07:43
Last Modified:27 Nov 2013 20:40
Publisher:Nature Publishing Group
Publisher DOI:10.1038/ncb2281
PubMed ID:21725319
Citations:Web of Science®. Times Cited: 62
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Scopus®. Citation Count: 65

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