Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive 

Akshay, S; Bertea, M; Hobbie, S N; Oettinghaus, B; Shcherbakov, D; Böttger, E C; Akbergenov, R (2011). Phylogenetic sequence variations in bacterial rRNA affect species-specific susceptibility to drugs targeting protein synthesis. Antimicrobial Agents and Chemotherapy, 55(9):4096-4102.

Full text not available from this repository.

Abstract

Antibiotics targeting the bacterial ribosome typically bind to highly conserved rRNA regions with only minor phylogenetic sequence variations. It is unclear whether these sequence variations affect antibiotic susceptibility or resistance development. To address this question, we have investigated the drug binding pockets of aminoglycosides and macrolides/ketolides. The binding site of aminoglycosides is located within helix 44 of the 16S rRNA (A site); macrolides/ketolides bind to domain V of the 23S rRNA (peptidyltransferase center). We have used mutagenesis of rRNA sequences in Mycobacterium smegmatis ribosomes to reconstruct the different bacterial drug binding sites and to study the effects of rRNA sequence variations on drug activity. Our results provide a rationale for differences in species-specific drug susceptibility patterns and species-specific resistance phenotypes associated with mutational alterations in the drug binding pocket.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Microbiology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2011
Deposited On:14 Sep 2011 09:46
Last Modified:27 Nov 2013 23:44
Publisher:American Society for Microbiology
ISSN:0066-4804
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:10.1128/AAC.01398-10
PubMed ID:21730122
Citations:Web of Science®. Times Cited: 1
Google Scholar™

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page