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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-49684

Dresch, C; Ackermann, M; Bernd, V; de Andrade Pereira, B; Shortman, K; Fraefel, C (2011). Thymic but not splenic CD8⁺ DCs can efficiently cross-prime T cells in the absence of licensing factors. European Journal of Immunology, 41(9):2544-2555.

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Abstract

Cross-presentation is an important mechanism to elicit both immune defenses and tolerance. Although only a few DC subsets possess the machinery required for cross-presentation, little is known about differences in cross-presenting capabilities of DCs belonging to the same subpopulation but localized in different lymphoid organs. In this study, we demonstrate that steady-state thymic CD8(+) DCs can efficiently cross-prime naïve CD8(+) T cells in the absence of costimulation. Surprisingly, cross-priming by splenic CD8(+) DCs was dependent on licensing factors such as GM-CSF. In the absence of GM-CSF, antigen-MHC-class-I complexes were detected on thymic but not on splenic CD8(+) DCs, indicating that the cross-presentation capacity of the thymic subpopulation was higher. The observed cross-priming differences between thymic and splenic CD8(+) DCs did not correlate with differential antigen capture or costimulatory molecules found on the surface of DCs. Moreover, we did not detect overall impairment of antigen presentation, as peptide-loaded splenic CD8(+) DCs were able to induce CD8(+) T-cell proliferation. The observation that thymic CD8(+) DCs are more efficient than splenic CD8(+) DCs in T-cell cross-priming in the absence of licensing factors indicates that the requirements for efficient antigen presentation differ between these cells.

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Virology
DDC:570 Life sciences; biology
Language:English
Date:26 August 2011
Deposited On:19 Sep 2011 16:00
Last Modified:04 Apr 2014 22:39
Publisher:Wiley-Blackwell
ISSN:0014-2980
Publisher DOI:10.1002/eji.201041374
PubMed ID:21748731
Citations:Web of Science®. Times Cited: 9
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