Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-49808
Oufir, M; Bisset, L R; Hoffmann, S R K; Xue, G; Klauser, S; Bergamaschi, B; Gervaix, A; Böni, J; Schüpbach, J; Gutte, B (2011). Artificial 64-residue HIV-1 enhancer-binding peptide is a potent inhibitor of viral replication in HIV-1-infected cells. Advances in Virology, 2011:165871.
An artificial HIV-1 enhancer-binding peptide was extended by nine consecutive arginine residues at the C-terminus and by the nuclear localization signal of SV40 large T antigen at the N-terminus. The resulting synthetic 64-residue peptide was found to bind to the two enhancers of the HIV-1 long terminal repeat, cross the plasma membrane and the nuclear envelope of human cells, and suppress the HIV-1 enhancer-controlled expression of a green fluorescent protein reporter gene. Moreover, HIV-1 replication is
inhibited by this peptide in HIV-1-infected CEM-GFP cells as revealed by HIV-1 p24 ELISA and real-time RT-PCR of HIV-1 RNA.
Rapid uptake of this intracellular stable and inhibitory peptide into the cells implies that this peptide may have the potential to attenuate HIV-1 replication in vivo.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Institute of Biochemistry|
07 Faculty of Science > Institute of Biochemistry
04 Faculty of Medicine > Institute of Medical Virology
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||28 Sep 2011 14:01|
|Last Modified:||23 Nov 2012 17:13|
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