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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-49847

Kristiansen, G; Hu, J; Wichmann, D; Stiehl, D P; Rose, M; Gerhardt, J; Bohnert, A; Ten Haaf, A; Moch, H; Raleigh, J; Varia, M A; Subarsky, P; Scandurra, F M; Gnaiger, E; Gleixner, E; Bicker, A; Gassmann, M; Hankeln, T; Dahl, E; Gorr, T A (2011). Endogenous myoglobin in breast cancer is hypoxia-inducible by alternative transcription and functions to impair mitochondrial activity: a role in tumor suppression? Journal of Biological Chemistry, 286(50):43417-43428.

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Abstract

Recently, immunohistochemical analysis of myoglobin (MB) in human breast cancer specimens has revealed a surprisingly widespread expression of MB in this non-muscle context. The positive correlation with hypoxia-inducible factor 2 and carbonic anhydrase IX suggested that oxygen regulates myoglobin expression in breast carcinomas. Here we report that MB mRNA and protein levels are robustly induced by prolonged hypoxia in breast cancer cell lines, in part via HIF-1/-2 dependent transactivation. The hypoxia-induced MB mRNA originated from a novel, alternative transcription start site 6 kb upstream of the ATG codon. MB regulation in normal and tumor tissue may thus be fundamentally different. Functionally, the knockdown of MB in MDA-MB468 breast cancer cells resulted in an unexpected increase of O2 uptake and elevated activities of mitochondrial enzymes during hypoxia. Silencing of MB transcription attenuated proliferation rates and motility capacities of hypoxic and, surprisingly, also fully oxygenated breast cancer cells. Endogenous MB in cancer cells is apparently involved in controlling oxidative cell energy metabolism, contrary to the mouse heart, where the targeted disruption of the Mb gene did not effect myocardial energetics and O2 consumption. This control function of MB seemingly impacts mitochondria and influences cell proliferation and motility, but it does so in ways not directly related to the facilitated diffusion or storage of O2. Hypothetically, myoglobins mitochondria-impairing role in hypoxic cancer cells is part of a novel tumor-suppressive function.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Surgical Pathology
04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology

04 Faculty of Medicine > Center for Integrative Human Physiology
05 Vetsuisse Faculty > Institute of Veterinary Physiology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2011
Deposited On:28 Sep 2011 13:12
Last Modified:28 Nov 2013 01:25
Publisher:American Society for Biochemistry and Molecular Biology
ISSN:0021-9258
Additional Information:This research was originally published in Journal of Biological Chemistry, Kristiansen, G [et al.], Endogenous myoglobin in breast cancer is hypoxia-inducible by alternative transcription and functions to impair mitochondrial activity: a role in tumor suppression?, 2011 © the American Society for Biochemistry and Molecular Biology.
Publisher DOI:10.1074/jbc.M111.227553
PubMed ID:21930697
Citations:Web of Science®. Times Cited: 8
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Scopus®. Citation Count: 5

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