Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-49885
Strunze, S; Engelke, M F; Wang, I-H; Puntener, D; Boucke, K; Schleich, S; Way, M; Schoenenberger, P; Burckhardt, C J; Greber, U F (2011). Kinesin-1-mediated capsid disassembly and disruption of the nuclear pore complex promote virus infection. Cell Host & Microbe, 10(3):210-223.
View at publisher
Many viruses deliver their genomes into the host cell nucleus for replication. However, the size restrictions of the nuclear pore complex (NPC), which regulates the passage of proteins, nucleic acids, and solutes through the nuclear envelope, require virus capsid uncoating before viral DNA can access the nucleus. We report a microtubule motor kinesin-1-mediated and NPC-supported mechanism of adenovirus uncoating. The capsid binds to the NPC filament protein Nup214 and kinesin-1 light-chain Klc1/2. The nucleoporin Nup358, which is bound to Nup214/Nup88, interacts with the kinesin-1 heavy-chain Kif5c to indirectly link the capsid to the kinesin motor. Kinesin-1 disrupts capsids docked at Nup214, which compromises the NPC and dislocates nucleoporins and capsid fragments into the cytoplasm. NPC disruption increases nuclear envelope permeability as indicated by the nuclear influx of large cytoplasmic dextran polymers. Thus, kinesin-1 uncoats viral DNA and compromises NPC integrity, allowing viral genomes nuclear access to promote infection.
228 downloads since deposited on 10 Oct 2011
75 downloads since 12 months
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||07 Faculty of Science > Institute of Molecular Life Sciences|
|DDC:||570 Life sciences; biology|
|Uncontrolled Keywords:||kinesin; DNA tumor virus; nuclear pore complex; Nup214; Nup358; nuclear import; virus uncoating; disassembly; microtubule; motor protein; DNA import; nuclear transport; heavy chain; light chain; protein IX;|
|Date:||15 September 2011|
|Deposited On:||10 Oct 2011 15:33|
|Last Modified:||30 Nov 2013 01:21|
Users (please log in): suggest update or correction for this item
Repository Staff Only: item control page