Trajkovic, K; Hsu, C; Chiantia, S; Rajendran, L; Wenzel, D; Wieland, F; Schwille, P; Brügger, B; Simons, M (2008). Ceramide triggers budding of exosome vesicles into multivesicular endosomes. Science, 319(5867):1244-1247.
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Intraluminal vesicles of multivesicular endosomes are either sorted for cargo degradation into lysosomes or secreted as exosomes into the extracellular milieu. The mechanisms underlying the sorting of membrane into the different populations of intraluminal vesicles are unknown. Here, we find that cargo is segregated into distinct subdomains on the endosomal membrane and that the transfer of exosome-associated domains into the lumen of the endosome did not depend on the function of the ESCRT (endosomal sorting complex required for transport) machinery, but required the sphingolipid ceramide. Purified exosomes were enriched in ceramide, and the release of exosomes was reduced after the inhibition of neutral sphingomyelinases. These results establish a pathway in intraendosomal membrane transport and exosome formation.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Psychiatric University Hospital Zurich > Division of Psychiatric Research and Clinic for Psychogeriatric Medicine|
|DDC:||610 Medicine & health|
|Deposited On:||19 Oct 2011 12:10|
|Last Modified:||28 Nov 2013 01:20|
|Publisher:||American Association for the Advancement of Science (AAAS)|
|Citations:||Web of Science®. Times Cited: 380|
Scopus®. Citation Count: 409
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