Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive 

Siegel, G; Obernosterer, G; Fiore, R; Oehmen, M; Bicker, S; Christensen, M; Khudayberdiev, S; Leuschner, P F; Busch, C J L; Kane, C; Hübel, K; Dekker, F; Hedberg, C; Rengarajan, B; Drepper, C; Waldmann, H; Kauppinen, S; Greenberg, M E; Draguhn, A; Rehmsmeier, M; Martinez, J; Schratt, G M (2009). A functional screen implicates microRNA-138-dependent regulation of the depalmitoylation enzyme APT1 in dendritic spine morphogenesis. Nature Cell Biology, 11(6):705-716.

Full text not available from this repository.

Abstract

The microRNA pathway has been implicated in the regulation of synaptic protein synthesis and ultimately in dendritic spine morphogenesis, a phenomenon associated with long-lasting forms of memory. However, the particular microRNAs (miRNAs) involved are largely unknown. Here we identify specific miRNAs that function at synapses to control dendritic spine structure by performing a functional screen. One of the identified miRNAs, miR-138, is highly enriched in the brain, localized within dendrites and negatively regulates the size of dendritic spines in rat hippocampal neurons. miR-138 controls the expression of acyl protein thioesterase 1 (APT1), an enzyme regulating the palmitoylation status of proteins that are known to function at the synapse, including the alpha(13) subunits of G proteins (Galpha(13)). RNA-interference-mediated knockdown of APT1 and the expression of membrane-localized Galpha(13) both suppress spine enlargement caused by inhibition of miR-138, suggesting that APT1-regulated depalmitoylation of Galpha(13) might be an important downstream event of miR-138 function. Our results uncover a previously unknown miRNA-dependent mechanism in neurons and demonstrate a previously unrecognized complexity of miRNA-dependent control of dendritic spine morphogenesis.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Psychiatric University Hospital Zurich > Division of Psychiatric Research and Clinic for Psychogeriatric Medicine
DDC:610 Medicine & health
Language:English
Date:2009
Deposited On:24 Oct 2011 17:04
Last Modified:27 Nov 2013 18:03
Publisher:Nature Publishing Group
ISSN:1465-7392
Publisher DOI:10.1038/ncb1876
PubMed ID:19465924
Citations:Web of Science®. Times Cited: 169
Google Scholar™

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page