Quick Search:

uzh logo
Browse by:

Zurich Open Repository and Archive

Hediger, M; Burghardt, G; Siegenthaler, C; Buser, N; Hilfiker-Kleiner, D; Dübendorfer, A; Bopp, D (2004). Sex determination in Drosophila melanogaster and Musca domestica converges at the level of the terminal regulator doublesex. Development Genes and Evolution, 214(1):29-42.

Full text not available from this repository.

View at publisher


Sex-determining cascades are supposed to have evolved in a retrograde manner from bottom to top. Wilkins' 1995 hypothesis finds support from our comparative studies in Drosophila melanogaster and Musca domestica, two dipteran species that separated some 120 million years ago. The sex-determining cascades in these flies differ at the level of the primary sex-determining signal and their targets, Sxl in Drosophila and F in Musca. Here we present evidence that they converge at the level of the terminal regulator, doublesex ( dsx), which conveys the selected sexual fate to the differentiation genes. The dsx homologue in Musca, Md-dsx, encodes male-specific (MdDSX(M)) and female-specific (MdDSX(F)) protein variants which correspond in structure to those in Drosophila. Sex-specific regulation of Md-dsx is controlled by the switch gene F via a splicing mechanism that is similar but in some relevant aspects different from that in Drosophila. MdDSX(F) expression can activate the vitellogenin genes in Drosophila and Musca males, and MdDSX(M) expression in Drosophila females can cause male-like pigmentation of posterior tergites, suggesting that these Musca dsx variants are conserved not only in structure but also in function. Furthermore, downregulation of Md-dsx activity in Musca by injecting dsRNA into embryos leads to intersexual differentiation of the gonads. These results strongly support a role of Md-dsx as the final regulatory gene in the sex-determining hierarchy of the housefly.


78 citations in Web of Science®
74 citations in Scopus®
Google Scholar™


Additional indexing

Item Type:Journal Article, refereed
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
Dewey Decimal Classification:570 Life sciences; biology
Date:1 January 2004
Deposited On:11 Feb 2008 12:15
Last Modified:05 Apr 2016 12:14
Publisher DOI:10.1007/s00427-003-0372-2
PubMed ID:14673649

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page