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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-50828

Weber, B; Schoenauer, R; Papadopulos, F; Modregger, P; Peter, S; Stampanoni, M; Mauri, A; Mazza, E; Gorelik, J; Agarkova, I; Frese, L; Breymann, C; Kretschmar, O; Hoerstrup, S P (2011). Engineering of living autologous human umbilical cord cell-based septal occluder membranes using composite PGA-P4HB matrices. Biomaterials, 32(36):9630-9641.

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Abstract

Interventional closure of intracardiac wall defects using occluder devices has evolved as a highly attractive treatment option. However, incomplete and delayed healing reactions often result in a major risk of residual defects, thromboembolism, or device fractures. Biodegradable living tissue engineered occluder membranes (TEOMs) could provide autologous thromboresistant implants with growth and remodeling capacities. PGA-P4HB composite matrices were seeded with human umbilical cord-derived cells or vascular-derived control cells and exposed to static (n = 19) or dynamic (n = 13) conditioning. Harvested TEOMs were integrated into occluder frameworks, exposed to crimping and delivered into pre-formed defects of juvenile porcine hearts. Dynamically conditioned TEOM constructs showed higher collagen formation in histology than static constructs with significantly higher stiffness moduli in uniaxial tensile testing. Grating interferometry revealed substantial but inhomogeneous cone-like degradation of the composite matrices in dynamic conditioning. The crimping and delivery procedures resulted in no significant changes in macroscopy, histo-morphology, cellular viability, DNA or hydroxyproline content, and scanning electron microscopy findings. Here, we present the in vitro fabrication, crimping and experimental delivery of living human umbilical cord-cell derived TEOMs based on composite matrices as a potential future autologous therapy of intracardiac wall defects.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Division of Surgical Research
04 Faculty of Medicine > University Hospital Zurich > Clinic for Obstetrics
04 Faculty of Medicine > University Children's Hospital Zurich > Medical Clinic
04 Faculty of Medicine > Institute of Biomedical Engineering
04 Faculty of Medicine > Center for Medical Research
04 Faculty of Medicine > University Hospital Zurich > Clinic for Cardiovascular Surgery
DDC:570 Life sciences; biology
170 Ethics
610 Medicine & health
Language:English
Date:2011
Deposited On:10 Nov 2011 18:16
Last Modified:28 Nov 2013 10:01
Publisher:Elsevier
ISSN:0142-9612
Publisher DOI:10.1016/j.biomaterials.2011.07.070
PubMed ID:21978890
Citations:Web of Science®. Times Cited: 2
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