Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-5088
Mullane, N R; O’Gaora, P; Nally, J E; Iversen, C; Whyte, P; Wall, P G; Fannning, S (2008). Molecular analysis of the Enterobacter sakazakii O-antigen gene locus. Applied and Environmental Microbiology, 74(12):3783-3794.
View at publisher
Nucleotide polymorphism associated with the O-antigen-encoding locus, rfb, in Enterobacter sakazakii was determined by PCR-restriction fragment length polymorphism analysis. Based on the analysis of these DNA profiles, 12 unique banding patterns were detected among a collection of 62 strains from diverse origins. Two common profiles were identified and were designated serotypes O:1 and O:2. DNA sequencing of the 12,500-bp region flanked by galF and gnd identified 11 open reading frames, all with the same transcriptional direction. Analysis of the proximal region of both sequences demonstrated remarkable heterogeneity. A PCR assay targeting genes specific for the two prominent serotypes was developed and applied for the identification of these strains recovered from food, environmental, and clinical samples.
63 downloads since deposited on 03 Dec 2008
16 downloads since 12 months
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||05 Vetsuisse Faculty > Institute of Food Safety and Hygiene|
|Dewey Decimal Classification:||570 Life sciences; biology
610 Medicine & health
|Deposited On:||03 Dec 2008 10:58|
|Last Modified:||27 Nov 2013 21:54|
|Publisher:||American Society for Microbiology|
|Additional Information:||Copyright: American Society for Microbiology|
Users (please log in): suggest update or correction for this item
Repository Staff Only: item control page