Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-5088
Mullane, N R; O’Gaora, P; Nally, J E; Iversen, C; Whyte, P; Wall, P G; Fannning, S (2008). Molecular analysis of the Enterobacter sakazakii O-antigen gene locus. Applied and Environmental Microbiology, 74(12):3783-3794.
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Nucleotide polymorphism associated with the O-antigen-encoding locus, rfb, in Enterobacter sakazakii was determined by PCR-restriction fragment length polymorphism analysis. Based on the analysis of these DNA profiles, 12 unique banding patterns were detected among a collection of 62 strains from diverse origins. Two common profiles were identified and were designated serotypes O:1 and O:2. DNA sequencing of the 12,500-bp region flanked by galF and gnd identified 11 open reading frames, all with the same transcriptional direction. Analysis of the proximal region of both sequences demonstrated remarkable heterogeneity. A PCR assay targeting genes specific for the two prominent serotypes was developed and applied for the identification of these strains recovered from food, environmental, and clinical samples.
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|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||05 Vetsuisse Faculty > Institute of Food Safety and Hygiene|
|DDC:||570 Life sciences; biology
610 Medicine & health
|Deposited On:||03 Dec 2008 10:58|
|Last Modified:||27 Nov 2013 21:54|
|Publisher:||American Society for Microbiology|
|Additional Information:||Copyright: American Society for Microbiology|
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