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Molecular analysis of the Enterobacter sakazakii O-antigen gene locus


Mullane, N R; O’Gaora, P; Nally, J E; Iversen, C; Whyte, P; Wall, P G; Fannning, S (2008). Molecular analysis of the Enterobacter sakazakii O-antigen gene locus. Applied and Environmental Microbiology, 74(12):3783-3794.

Abstract

Nucleotide polymorphism associated with the O-antigen-encoding locus, rfb, in Enterobacter sakazakii was determined by PCR-restriction fragment length polymorphism analysis. Based on the analysis of these DNA profiles, 12 unique banding patterns were detected among a collection of 62 strains from diverse origins. Two common profiles were identified and were designated serotypes O:1 and O:2. DNA sequencing of the 12,500-bp region flanked by galF and gnd identified 11 open reading frames, all with the same transcriptional direction. Analysis of the proximal region of both sequences demonstrated remarkable heterogeneity. A PCR assay targeting genes specific for the two prominent serotypes was developed and applied for the identification of these strains recovered from food, environmental, and clinical samples.

Nucleotide polymorphism associated with the O-antigen-encoding locus, rfb, in Enterobacter sakazakii was determined by PCR-restriction fragment length polymorphism analysis. Based on the analysis of these DNA profiles, 12 unique banding patterns were detected among a collection of 62 strains from diverse origins. Two common profiles were identified and were designated serotypes O:1 and O:2. DNA sequencing of the 12,500-bp region flanked by galF and gnd identified 11 open reading frames, all with the same transcriptional direction. Analysis of the proximal region of both sequences demonstrated remarkable heterogeneity. A PCR assay targeting genes specific for the two prominent serotypes was developed and applied for the identification of these strains recovered from food, environmental, and clinical samples.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:June 2008
Deposited On:03 Dec 2008 10:58
Last Modified:05 Apr 2016 12:32
Publisher:American Society for Microbiology
ISSN:0099-2240
Additional Information:Copyright: American Society for Microbiology
Publisher DOI:10.1128/AEM.02302-07
PubMed ID:18441119
Permanent URL: http://doi.org/10.5167/uzh-5088

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