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Development of multiple-locus variable-number tandem-repeat analysis for the molecular subtyping of Enterobacter sakazakii


Mullane, N R; Ryan, M; Iversen, C; Murphy, M; O’Gaora, P; Quinn, T; Whyte, P; Wall, P G; Fanning, C (2008). Development of multiple-locus variable-number tandem-repeat analysis for the molecular subtyping of Enterobacter sakazakii. Applied and Environmental Microbiology, 74(4):1223-1231.

Abstract

The genomic content of Enterobacter sakazakii strain ATCC BAA-894 was analyzed for variable-number tandem repeats (VNTRs). In this study we report the development of a multiple-locus VNTR analysis (MLVA) strategy for the subtyping of E. sakazakii. The method is based on a GeneScan analysis of four VNTR loci labeled with multiple fluorescent dyes. This approach was applied to a collection of 112 isolates representing all 16 of the currently defined E. sakazakii biogroups. MLVA successfully discriminated among these isolates and compared favorably with pulsed-field gel electrophoresis. The method was relatively fast and easy to perform. The potential value of MLVA as an epidemiological tool is discussed.

The genomic content of Enterobacter sakazakii strain ATCC BAA-894 was analyzed for variable-number tandem repeats (VNTRs). In this study we report the development of a multiple-locus VNTR analysis (MLVA) strategy for the subtyping of E. sakazakii. The method is based on a GeneScan analysis of four VNTR loci labeled with multiple fluorescent dyes. This approach was applied to a collection of 112 isolates representing all 16 of the currently defined E. sakazakii biogroups. MLVA successfully discriminated among these isolates and compared favorably with pulsed-field gel electrophoresis. The method was relatively fast and easy to perform. The potential value of MLVA as an epidemiological tool is discussed.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2008
Deposited On:03 Dec 2008 13:50
Last Modified:05 Apr 2016 12:32
Publisher:American Society for Microbiology
ISSN:0099-2240
Additional Information:Copyright: American Society for Microbiology
Publisher DOI:10.1128/AEM.01726-07
PubMed ID:18083860
Permanent URL: http://doi.org/10.5167/uzh-5089

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