Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-51058
Bramlage, C P; Müller, G A; Tampe, B; Bevanda, J; Maatouk, I; Koziolek, M; Lange, K; Ahrens, K; Schmid, H; Cohen, C D; Bramlage, P; Kretzler, M; Strutz, F (2011). The role of bone morphogenetic protein-5 (BMP-5) in human nephrosclerosis. Journal of Nephrology, 24(5):647-654.
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Background: Bone morphogenetic protein-5 (BMP-5)
has been shown to be essential for nephrogenesis. Its
role in adult kidney and in patients with hypertensive
nephrosclerosis is still unknown.
Methods: BMP-5 expression was evaluated by immunostaining
and real-time PCR in tissue samples from
normal and nephrosclerotic human kidneys. The impact
of transforming growth factor-β (TGF-β), tumor
necrosis factor-α (TNF-α) and angiotensin-II (AT-II) on
expression of BMP-5 and its receptors was quantified
in proximal tubular cells (HK-2). Functional characteristics
of BMP-5 were evaluated by testing its influence
on TGF-β–induced epithelial-to-mesenchymal transition
(EMT), TNF-α–induced apoptosis of HK-2 cells
and inflammatory cell infiltration.
Results: BMP-5 expression was localized in tubular
epithelial cells and significantly decreased in nephrosclerotic
kidneys. Stimulation of HK-2 cells with TGF-β,
TNF-α and AT-II resulted in a significant decreased expression
of BMP-5 and its receptors. BMP-5 attenuated
TGF-β–induced EMT, TNF-α–induced apoptosis
and migration of mononuclear cells.
Conclusions: BMP-5 is expressed in the tubuli of adult
kidneys. Its decreased expression in nephrosclerosis
along with its regenerative capabilities in HK-2 cells
may point to a protective role in hypertensive nephrosclerosis.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Clinic for Nephrology|
04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||17 Nov 2011 11:17|
|Last Modified:||09 Dec 2012 18:56|
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