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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-51306

Wandeler, P; Camenisch, G (2011). Identifying Y-chromosomal diversity by long-template PCR. Molecular Ecology Resources, 11(5):835-841.

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Abstract

Comparing Y-chromosomal and mitochondrial haplotype variation is a promising approach to independently investigate paternal and maternal evolutionary histories in wild mammal populations. However, the difficulty of developing male-specific genetic markers, because of its distinctive genetic architecture and the general low level of polymorphisms observed on the Y chromosome, hampers usually an effective application of this approach. Here, we present a further method of the established Y chromosome conserved anchored tagged sequences strategy to develop Y-chromosomal markers by screening introns of male-specific region (MSY) genes for sequence polymorphisms. By applying long-template PCR using target species-specific primers, adequate sequence information of several kb in size can be obtained. We applied this method in the snow vole (Chionomys nivalis) and obtained 12.4 kb of male-specific sequence data for nine males representing four populations in the Swiss Alps. A total of 28 single nucleotide polymorphisms, four indels (>1 bp) and one polymorphic microsatellite were identified in introns of the SMCY and DBY genes. Based on this information, we developed a Y-chromosomal genotyping assay and identified four different paternal lineages within one local snow vole population. The method we present is straightforward and as such will probably be suitable to detect adequate Y-chromosomal diversity in a wide range of mammalian species.

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Evolutionary Biology and Environmental Studies
DDC:570 Life sciences; biology
590 Animals (Zoology)
Language:English
Date:September 2011
Deposited On:25 Nov 2011 11:42
Last Modified:03 Dec 2013 17:36
Publisher:Wiley-Blackwell
ISSN:1755-098X
Publisher DOI:10.1111/j.1755-0998.2011.03013.x
PubMed ID:21457481
Other Identification Number:ISI:000293915800012
Citations:Web of Science®
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