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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-51317

Takizawa, H; Regoes, R R; Boddupalli, CS; Bonhoeffer , S; Manz, M G (2011). Dynamic variation in cycling of hematopoietic stem cells in steady state and inflammation. Journal of Experimental Medicine, 208(2):273-284.

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Abstract

Hematopoietic stem cells (HSCs) maintain blood production. How often mouse HSCs divide and whether each HSC contributes simultaneously, sequentially, or repetitively to hematopoiesis remains to be determined. We track division of 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled HSC in vivo. We found that, in steady-state mice, bone marrow cells capable of reconstituting lifelong hematopoiesis are found within both fast-cycling (undergoing five or more divisions in 7 wk) and quiescent (undergoing zero divisions in 12-14 wk) lineage marker-negative c-Kit(+) Sca-1(+) populations. The contribution of each population to hematopoiesis can fluctuate with time, and cells with extensive proliferative history are prone to return to quiescence. Furthermore, injection of the bacterial component lipopolysaccharide increased the proliferation and self-renewal capacity of HSCs. These findings suggest a model in which all HSCs undergo dynamic and demand-adapted entry into and exit out of the cell cycle over time. This may facilitate a similar degree of turnover of the entire HSC pool at the end of life.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Hematology
DDC:610 Medicine & health
Language:English
Date:14 February 2011
Deposited On:25 Nov 2011 12:21
Last Modified:23 Nov 2012 16:45
Publisher:Rockefeller University Press
ISSN:0022-1007
Additional Information:Copyright: the Authors
Publisher DOI:10.1084/jem.20101643
PubMed ID:21300914
Citations:Google Scholar™
Scopus®. Citation Count: 52

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