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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-53204

Pernot, E; Terryn, S; Cheong, S C; Markadieu, N; Janas, S; Blockmans, M; Jacoby, M; Pouillon, V; Gayral, S; Rossier, B C; Beauwens, R; Erneux, C; Devuyst, O; Schurmans, S (2011). The inositol Inpp5k 5-phosphatase affects osmoregulation through the vasopressin-aquaporin 2 pathway in the collecting system. Pflügers Archiv: European Journal of Physiology (Pflugers Archiv), 462(6):871-883.

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Abstract

Inositol Inpp5k (or Pps, SKIP) is a member of the inositol polyphosphate 5-phosphatases family with a poorly characterized function in vivo. In this study, we explored the function of this inositol 5-phosphatase in mice and cells overexpressing the 42-kDa mouse Inpp5k protein. Inpp5k transgenic mice present defects in water metabolism characterized by a reduced plasma osmolality at baseline, a delayed urinary water excretion following a water load, and an increased acute response to vasopressin. These defects are associated with the expression of the Inpp5k transgene in renal collecting ducts and with alterations in the arginine vasopressin/aquaporin-2 signalling pathway in this tubular segment. Analysis in a mouse collecting duct mCCD cell line revealed that Inpp5k overexpression leads to increased expression of the arginine vasopressin receptor type 2 and increased cAMP response to arginine vasopressin, providing a basis for increased aquaporin-2 expression and plasma membrane localization with increased osmotically induced water transport. Altogether, our results indicate that Inpp5k 5-phosphatase is important for the control of the arginine vasopressin/aquaporin-2 signalling pathway and water transport in kidney collecting ducts.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology

04 Faculty of Medicine > Center for Integrative Human Physiology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2011
Deposited On:11 Jan 2012 12:31
Last Modified:07 Dec 2013 10:05
Publisher:Springer
ISSN:0031-6768
Additional Information:The original publication is available at www.springerlink.com
Publisher DOI:10.1007/s00424-011-1028-0
PubMed ID:21938401
Citations:Web of Science®. Times Cited: 2
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Scopus®. Citation Count: 2

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