Sugihara, K; Delai, M; Szendro, I; Guillaume-Gentil, O; Vörös, J; Zambelli, T (2012). Simultaneous OWLS and EIS monitoring of supported lipid bilayers with the pore forming peptide melittin. Sensors and Actuators B: Chemical, 161(1):600-606.
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A simultaneous optical waveguide lightmode spectroscopy (OWLS) and electrochemical impedance spectroscopy (EIS) measurement was carried out for the investigation of a supported lipid bilayer and its interactions with a pore-forming peptide, melittin. It was achieved only after the optimization of the ITO coating on the waveguide to increase the electrical sensitivity and the functionalization of the waveguide with a polyelectrolyte to form a lipid bilayer over the ITO surface. The combined system enabled monitoring of melittin pore activities in a wider range of melittin concentrations than either technique alone (1 μg/ml < Cmelittin < 200 μg/ml). Furthermore, it provided unique information that could not be obtained by the individual methods, such as a better identification of the melittin-pore formation and an insight about the correlation between the total pore area vs. adsorbed amount of melittin.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Institute of Biomedical Engineering|
610 Medicine & health
|Deposited On:||13 Jan 2012 11:19|
|Last Modified:||23 Nov 2012 15:00|
|Additional Information:||Optical waveguide lightmode spectroscopy; Electrochemical impedance spectroscopy; Supported lipid bilayer; Polyelectrolyte; Melittin|
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