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Sugihara, K; Delai, M; Szendro, I; Guillaume-Gentil, O; Vörös, J; Zambelli, T (2012). Simultaneous OWLS and EIS monitoring of supported lipid bilayers with the pore forming peptide melittin. Sensors and Actuators B: Chemical, 161(1):600-606.

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Abstract

A simultaneous optical waveguide lightmode spectroscopy (OWLS) and electrochemical impedance spectroscopy (EIS) measurement was carried out for the investigation of a supported lipid bilayer and its interactions with a pore-forming peptide, melittin. It was achieved only after the optimization of the ITO coating on the waveguide to increase the electrical sensitivity and the functionalization of the waveguide with a polyelectrolyte to form a lipid bilayer over the ITO surface. The combined system enabled monitoring of melittin pore activities in a wider range of melittin concentrations than either technique alone (1 μg/ml < Cmelittin < 200 μg/ml). Furthermore, it provided unique information that could not be obtained by the individual methods, such as a better identification of the melittin-pore formation and an insight about the correlation between the total pore area vs. adsorbed amount of melittin.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Biomedical Engineering
DDC:170 Ethics
610 Medicine & health
Language:English
Date:2012
Deposited On:13 Jan 2012 10:19
Last Modified:27 Nov 2013 18:47
Publisher:Elsevier
ISSN:0925-4005
Additional Information: Optical waveguide lightmode spectroscopy; Electrochemical impedance spectroscopy; Supported lipid bilayer; Polyelectrolyte; Melittin
Publisher DOI:10.1016/j.snb.2011.11.007
Citations:Web of Science®. Times Cited: 2
Google Scholar™
Scopus®. Citation Count: 3

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