Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-53837

Tohge, T; Ramos, M S; Nunes-Nesi, A; Mutwil, M; Giavalisco, P; Steinhauser, D; Schellenberg, M; Willmitzer, L; Persson, S; Martinoia, E; Fernie, A R (2011). Toward the storage metabolome: profiling the barley vacuole. Plant Physiology, 157(3):1469-82.

[img]
Preview
Published Version
PDF
1MB

View at publisher
[img]
Preview
Supplemental Material
PDF (Supplemental Figures 1-3)
1MB
[img]Supplemental Material
Other (Supplemental Table I )
102kB
[img]Supplemental Material
Other (Supplemental Table II )
130kB

Abstract

While recent years have witnessed dramatic advances in our capacity to identify and quantify an ever-increasing number of plant metabolites, our understanding of how metabolism is spatially regulated is still far from complete. In an attempt to partially address this question, we studied the storage metabolome of the barley (Hordeum vulgare) vacuole. For this purpose, we used highly purified vacuoles isolated by silicon oil centrifugation and compared their metabolome with that found in the mesophyll protoplast from which they were derived. Using a combination of gas chromatography-mass spectrometry and Fourier transform-mass spectrometry, we were able to detect 59 (primary) metabolites for which we know the exact chemical structure and a further 200 (secondary) metabolites for which we have strong predicted chemical formulae. Taken together, these metabolites comprise amino acids, organic acids, sugars, sugar alcohols, shikimate pathway intermediates, vitamins, phenylpropanoids, and flavonoids. Of the 259 putative metabolites, some 12 were found exclusively in the vacuole and 34 were found exclusively in the protoplast, while 213 were common in both samples. When analyzed on a quantitative basis, however, there is even more variance, with more than 60 of these compounds being present above the detection limit of our protocols. The combined data were also analyzed with respect to the tonoplast proteome in an attempt to infer specificities of the transporter proteins embedded in this membrane. Following comparison with recent observations made using nonaqueous fractionation of Arabidopsis (Arabidopsis thaliana), we discuss these data in the context of current models of metabolic compartmentation in plants.

Citations

32 citations in Web of Science®
36 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

159 downloads since deposited on 03 Jan 2012
102 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Plant Biology
DDC:580 Plants (Botany)
Date:2011
Deposited On:03 Jan 2012 18:24
Last Modified:23 May 2014 22:10
Publisher:American Society of Plant Physiologists
ISSN:0032-0889
Publisher DOI:10.1104/pp.111.185710
PubMed ID:21949213

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page