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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-53850

Nirschl, M; Reuter, F; Vörös, J (2011). Review of transducer principles for label-free biomolecular interaction analysis. Biosensors, 1(3):70-92.

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Abstract

Label-free biomolecular interaction analysis is an important technique to study the chemical binding between e.g., protein and protein or protein and small molecule in real-time. The parameters obtained with this technique, such as the affinity, are important for drug development. While the surface plasmon resonance (SPR) instruments are most widely used, new types of sensors are emerging. These developments are generally driven by the need for higher throughput, lower sample consumption or by the need of complimentary information to the SPR data. This review aims to give an overview about a wide range of sensor transducers, the working principles and the peculiarities of each technology, e.g., concerning the set-up, sensitivity, sensor size or required sample volume. Starting from optical technologies like the SPR and waveguide based sensors, acoustic sensors like the quartz crystal microbalance (QCM) and the film bulk acoustic resonator (FBAR), calorimetric and electrochemical sensors are covered. Technologies long established in the market are presented together with those newly commercially available and with technologies in the early development stage. Finally, the commercially available instruments are summarized together with their sensitivity and the number of sensors usable in parallel and an outlook for potential future developments is given.

Item Type:Journal Article, not refereed, further contribution
Communities & Collections:04 Faculty of Medicine > Institute of Biomedical Engineering
DDC:170 Ethics
610 Medicine & health
Language:English
Date:2011
Deposited On:12 Jan 2012 17:18
Last Modified:24 Nov 2012 12:37
Publisher:Molecular Diversity Preservation International (MDPI)
ISSN:2079-6374
Publisher DOI:10.3390/bios1030070
Citations:Google Scholar™
Scopus®. Citation Count: 13

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