Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-54894
Grewe, B F; Voigt, F F; van 't Hoff, M; Helmchen, F (2011). Fast two-layer two-photon imaging of neuronal cell populations using an electrically tunable lens. Biomedical Optics Express, 2(7):2035-2046.
Functional two-photon Ca(2+)-imaging is a versatile tool to study the dynamics of neuronal populations in brain slices and living animals. However, population imaging is typically restricted to a single two-dimensional image plane. By introducing an electrically tunable lens into the excitation path of a two-photon microscope we were able to realize fast axial focus shifts within 15 ms. The maximum axial scan range was 0.7 mm employing a 40x NA0.8 water immersion objective, plenty for typically required ranges of 0.2-0.3 mm. By combining the axial scanning method with 2D acousto-optic frame scanning and random-access scanning, we measured neuronal population activity of about 40 neurons across two imaging planes separated by 40 μm and achieved scan rates up to 20-30 Hz. The method presented is easily applicable and allows upgrading of existing two-photon microscopes for fast 3D scanning.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Brain Research Institute|
Special Collections > SystemsX.ch
Special Collections > SystemsX.ch > Research, Technology and Development Projects > Neurochoice
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||17 Jan 2012 16:50|
|Last Modified:||06 Dec 2012 06:23|
|Publisher:||Optical Society of America (OSA)|
|Additional Information:||This paper was published in Biomedical Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/abstract.cfm?URI=boe-2-7-2035. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.|
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