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Leaf press juice from Bryophyllum pinnatum (Lamarck) Oken induces myometrial relaxation


Wächter, R; Brenneisen, R; Hamburger, M; Mennet, M; Schnelle, M; Worel, A M; Simões-Wüst, A P; von Mandach, U (2011). Leaf press juice from Bryophyllum pinnatum (Lamarck) Oken induces myometrial relaxation. Phytomedicine, 19(1):74-82.

Abstract

Aims:

The use of preparations from Bryophyllum pinnatum (Lamarck) Oken (Kalanchoe pinnata (Lamarck) Persoon) in tocolysis is supported by clinical evidence. We studied here the effect of B. pinnatum leaf press juice and its chemical fractions on the response of human myometrial strips. No data are available if the influence on myometrial strips of the juice differs from that of its components in the chemical fractions, in order to increase the pharmacological effect.
Methodology:

In vitro study to test the effect of repeated addition of B. pinnatum leaf press juice (BPJ) and its chemical components in several dilutions (undiluted, 1–10%) on myometrium strips hang up in a myograph chamber. Chemical analysis is including HPLC, MPLC with Sephadex LH-20 and TLC.
Results:

All test solutions are inhibiting contractility by reducing the amplitude and the area under the curve (AUC) of the contractions. Undiluted BPJ and its undiluted chemical fraction 4 are reducing most effective these two parameters: the amplitude was at 78% of the baseline (95% CI (77–89); p < 0.05) at the second addition of the BPJ and at 70% (95% CI (50–90); p < 0.05) of the first addition of fraction 4; the AUC was at 82% (95% CI (69–95); p < 0.05) of the baseline at the first addition of the press juice and at 51% (95% CI (27–74); p < 0.05) at the first addition of fraction 4. The BPJ decreased amplitude and AUC significantly faster and increased frequency significantly faster than the control. Fractions could be tentatively assigned to bufadienolids, flavonoids and cinnamic acids. Fraction 4, accounted for flavonoids, increased the frequency of the contractions most effectively: 557% of the baseline (95% CI (316–797); p < 0.05) at the first addition.
Conclusion:

Leaf juice of B. pinnatum and its flavonoid fraction are most effective in relaxing myometrial strips by inducing frequency.

Aims:

The use of preparations from Bryophyllum pinnatum (Lamarck) Oken (Kalanchoe pinnata (Lamarck) Persoon) in tocolysis is supported by clinical evidence. We studied here the effect of B. pinnatum leaf press juice and its chemical fractions on the response of human myometrial strips. No data are available if the influence on myometrial strips of the juice differs from that of its components in the chemical fractions, in order to increase the pharmacological effect.
Methodology:

In vitro study to test the effect of repeated addition of B. pinnatum leaf press juice (BPJ) and its chemical components in several dilutions (undiluted, 1–10%) on myometrium strips hang up in a myograph chamber. Chemical analysis is including HPLC, MPLC with Sephadex LH-20 and TLC.
Results:

All test solutions are inhibiting contractility by reducing the amplitude and the area under the curve (AUC) of the contractions. Undiluted BPJ and its undiluted chemical fraction 4 are reducing most effective these two parameters: the amplitude was at 78% of the baseline (95% CI (77–89); p < 0.05) at the second addition of the BPJ and at 70% (95% CI (50–90); p < 0.05) of the first addition of fraction 4; the AUC was at 82% (95% CI (69–95); p < 0.05) of the baseline at the first addition of the press juice and at 51% (95% CI (27–74); p < 0.05) at the first addition of fraction 4. The BPJ decreased amplitude and AUC significantly faster and increased frequency significantly faster than the control. Fractions could be tentatively assigned to bufadienolids, flavonoids and cinnamic acids. Fraction 4, accounted for flavonoids, increased the frequency of the contractions most effectively: 557% of the baseline (95% CI (316–797); p < 0.05) at the first addition.
Conclusion:

Leaf juice of B. pinnatum and its flavonoid fraction are most effective in relaxing myometrial strips by inducing frequency.

Citations

12 citations in Web of Science®
14 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Obstetrics
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:2011
Deposited On:06 Mar 2012 12:36
Last Modified:05 Apr 2016 15:30
Publisher:Elsevier
ISSN:0944-7113
Publisher DOI:10.1016/j.phymed.2011.06.032
PubMed ID:21802930
Permanent URL: http://doi.org/10.5167/uzh-57324

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