Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive 

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-57361

Hoogewijs, D; Ebner, B; Germani, F; Hoffmann, F G; Fabrizius, A; Moens, L; Burmester, T; Dewilde, S; Storz, J F; Vinogradov, S N; Hankeln, T (2012). Androglobin: a chimeric globin in metazoans that is preferentially expressed in mammalian testes. Molecular Biology and Evolution, 29(4):1105-1114.

[img]
Preview
Published Version
PDF
759kB

Abstract

Comparative genomic studies have led to the recent identification of several novel globin types in the Metazoa. They have revealed a surprising evolutionary diversity of functions beyond the familiar O2 supply roles of hemoglobin and myoglobin. Here we report the discovery of a hitherto unrecognized family of proteins with a unique modular architecture, possessing an N-terminal calpain-like domain, an internal, circular permuted globin domain, and an IQ calmodulin-binding motif. Putative orthologs are present in the genomes of many metazoan taxa, including vertebrates.
The calpain-like region is homologous to the catalytic domain II of the large subunit of human calpain-7. The globin domain satisfies the criteria of a myoglobin-like fold but is rearranged and split into two parts. The recombinantly expressed human globin domain exhibits an absorption spectrum characteristic of hexacoordination of the heme iron atom. Molecular evolutionary analyses indicate that this chimeric globin family is phylogenetically ancient and originated in the common ancestor to animals and choanoflagellates. In humans and mice, the gene is predominantly expressed in testis tissue, and we propose the name ‘‘androglobin’’ (Adgb). Expression is associated with postmeiotic stages of spermatogenesis and is insensitive to experimental hypoxia. Evidence exists for increased gene expression in fertile compared with infertile males.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology

04 Faculty of Medicine > Center for Integrative Human Physiology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2012
Deposited On:01 Feb 2012 21:34
Last Modified:09 Dec 2013 23:20
Publisher:Oxford University Press
ISSN:0737-4038
Funders:Marie Curie IEF Fellowship from the EU, Center for Computational Sciences, SRFN, Fund for Scientific Research, National Institute of Health, National Science Foundation
Publisher DOI:10.1093/molbev/msr246
PubMed ID:22115833
Citations:Web of Science®. Times Cited: 14
Google Scholar™
Scopus®. Citation Count: 15

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page