Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-57361
Hoogewijs, D; Ebner, B; Germani, F; Hoffmann, F G; Fabrizius, A; Moens, L; Burmester, T; Dewilde, S; Storz, J F; Vinogradov, S N; Hankeln, T (2012). Androglobin: a chimeric globin in metazoans that is preferentially expressed in mammalian testes. Molecular Biology and Evolution, 29(4):1105-1114.
Comparative genomic studies have led to the recent identification of several novel globin types in the Metazoa. They have revealed a surprising evolutionary diversity of functions beyond the familiar O2 supply roles of hemoglobin and myoglobin. Here we report the discovery of a hitherto unrecognized family of proteins with a unique modular architecture, possessing an N-terminal calpain-like domain, an internal, circular permuted globin domain, and an IQ calmodulin-binding motif. Putative orthologs are present in the genomes of many metazoan taxa, including vertebrates.
The calpain-like region is homologous to the catalytic domain II of the large subunit of human calpain-7. The globin domain satisfies the criteria of a myoglobin-like fold but is rearranged and split into two parts. The recombinantly expressed human globin domain exhibits an absorption spectrum characteristic of hexacoordination of the heme iron atom. Molecular evolutionary analyses indicate that this chimeric globin family is phylogenetically ancient and originated in the common ancestor to animals and choanoflagellates. In humans and mice, the gene is predominantly expressed in testis tissue, and we propose the name ‘‘androglobin’’ (Adgb). Expression is associated with postmeiotic stages of spermatogenesis and is insensitive to experimental hypoxia. Evidence exists for increased gene expression in fertile compared with infertile males.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Institute of Physiology|
07 Faculty of Science > Institute of Physiology
04 Faculty of Medicine > Center for Integrative Human Physiology
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||01 Feb 2012 22:34|
|Last Modified:||06 Dec 2012 19:48|
|Publisher:||Oxford University Press|
|Funders:||Marie Curie IEF Fellowship from the EU, Center for Computational Sciences, SRFN, Fund for Scientific Research, National Institute of Health, National Science Foundation|
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