Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-57607
Li, Qiang; Seeger, Stefan (2011). Multidonor Deep-UV FRET study of Protein–Ligand binding and its potential to obtain structure information. Journal of Physical Chemistry B, 115(46):13643-13649.
View at publisher
PDF - Registered users only
Fluorescence resonance energy transfer (FRET) using biotinylated β-galactosidase (βGAL) as donor and Alexa Fluor 350 (AF350) labelled avidin as acceptor has been investigated by means of steady-state fluorescence and time-resolved fluorescence spectroscopy. The donors are readily paired with acceptors through the well-established binding affinity of biotin and avidin. The fluorescence energy transfer efficiency was determined by the donor fluorescence emission and lifetime changes in the presence and absence of acceptor. The theoretical energy transfer efficiency and theoretical average distance between donor and acceptor after non-covalent binding was calculated by taking the distribution of tryptophan residues in βGAL and avidin as well as the location of AF350 in avidin into account, which agree with the experimental data. It is shown how information of the location of acceptor can be obtained. Further, the fluorescence intensity image of AF350 on biotinylated βGAL coated quartz surface through UV FRET has been recorded using deep UV laser-based fluorescence lifetime microscopy. The results demonstrate that (a) deep UV laser-based fluorescence lifetime microscopy is a simple and useful method to study UV FRET of proteins using intrinsic fluorescence, (b) structural information even in complex multi-donor systems can be obtained and (c) FRET signals can be obtained to detect binding events using the native fluorescence of proteins as multi-donor systems.
50 downloads since deposited on 05 Mar 2012
17 downloads since 12 months
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||07 Faculty of Science > Department of Chemistry|
|Dewey Decimal Classification:||540 Chemistry|
|Deposited On:||05 Mar 2012 07:59|
|Last Modified:||10 Apr 2015 12:14|
|Publisher:||American Chemical Society|
|Additional Information:||This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Physical Chemistry B, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/jp2035289.|
Users (please log in): suggest update or correction for this item
Repository Staff Only: item control page