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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-58320

Kaufmann, C; Ziegler, D; Schaffner, F; Carpenter, S; Pflüger, V; Mathis, A (2011). Evaluation of matrix-assisted laser desorption/ionization time of flight mass spectrometry for characterization of Culicoides nubeculosus biting midges. Medical and Veterinary Entomology, 25(1):32-38.

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Abstract

Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has shown promise in species identification of insect species. We evaluated its potential to consistently characterize laboratory-reared biting midges of the species Culicoides nubeculosus (Meigen) (Diptera: Ceratopogonidae). Twenty-one reproducible potential biomarker masses for C. nubeculosus were identified under different experimental treatments. These treatments included the homogenization of insects in either water or known concentrations of formic acid. The biomarker masses were present independent of age, gender and different periods of storage of individuals in 70% ethanol (a standard preservation method). It was found that the presence of blood in females reduced the intensity of the MALDI-TOF pattern, necessitating the removal of the abdomen before analysis. The protein profiles of a related non-biting midge, Forcipomyia sp. (Diptera: Ceratopogonidae), and of Aedes japonicus japonicus (Theobald) (Diptera: Culicidae) mosquitoes were also examined and were distinctly different. These findings provide preliminary data to optimize future studies in differentiation of species within the Culicoides genus using MALDI-TOF MS which is a rapid, simple, reliable and cost-effective technique.

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Parasitology
04 Faculty of Medicine > Institute of Parasitology
DDC:570 Life sciences; biology
610 Medicine & health
600 Technology
Language:English
Date:2011
Deposited On:07 Mar 2012 11:16
Last Modified:08 Dec 2013 04:16
Publisher:Wiley-Blackwell
ISSN:0269-283X
Publisher DOI:10.1111/j.1365-2915.2010.00927.x
PubMed ID:21118284
Citations:Web of Science®. Times Cited: 19
Google Scholar™
Scopus®. Citation Count: 18

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