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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-58619

Schneider, H; Errede, M; Ulrich, N H; Virgintino, D; Frei, K; Bertalanffy, H (2011). Impairment of tight junctions and glucose transport in endothelial cells of human cerebral cavernous malformations. Journal of Neuropathology and Experimental Neurology, 70(6):417-429.

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Abstract

Cerebral cavernous malformations (CCMs) often cause hemorrhages that can result in severe clinical manifestations, including hemiparesis and seizures. The underlying mechanisms of the aggressive behavior of CCMs are undetermined to date, but alterations of vascular matrix components may be involved. We compared the localization of the tight junction proteins (TJPs) in 12 CCM specimens and the expression of glucose transporter 1 (GLUT-1), which is sensitive to alterations in TJP levels, in 5 CCM specimens with those in 5 control temporal lobectomy specimens without CCM by immunofluorescence microscopy. The TJPs occludin, claudin-5, and zonula occludens ZO-1 were downregulated at intercellular contact sites and partly redistributed within the surrounding tissue in the CCM samples; there was also a marked reduction of GLUT-1 immunoreactivity compared with that in control specimens. Corresponding analysis using quantitative real-time reverse transcription polymerase chain reaction on 8 CCM and 8 control specimens revealed significant downregulation of mRNA expression of occludin, claudin-5, ZO-1, and GLUT-1. The altered expression and localization of the TJPs at interendothelial contact sites accompanied by a reduction of GLUT-1 expression in dilated CCM microvessels likely affect vascular matrix stability and may contribute to hemorrhages of CCMs.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Neurology
04 Faculty of Medicine > University Hospital Zurich > Clinic for Neurosurgery
DDC:610 Medicine & health
Language:English
Date:June 2011
Deposited On:11 Feb 2012 19:30
Last Modified:29 Nov 2013 12:39
Publisher:Lippincott Williams and Wilkins
ISSN:0022-3069 (P) 1554-6578 (E)
Publisher DOI:10.1097/NEN.0b013e31821bc40e
PubMed ID:21572340
Citations:Web of Science®. Times Cited: 6
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Scopus®. Citation Count: 6

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