Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-588
Ryder, E; Blows, F; Ashburner, M; Bautista-Llacer, R; Coulson, D; Drummond, Jenny; Webster, J; Gubb, D; Gunton, N; Johnson, G; O'Kane, C J; Huen, D; Sharma, P; Asztalos, Z; Baisch, H; Schulze, J; Kube, M; Kittlaus, K; Reuter, G; Maroy, P; Szidonya, J; Rasmuson-Lestander, A; Ekström, K; Dickson, B J; Hugentobler, C; Stocker, H; Hafen, E; Lepesant, J A; Pflugfelder, G O; Heisenberg, M; Mechler, B; Serras, F; Corominas, M; Schneuwly, S; Preat, T; Roote, J; Russell, S (2004). The DrosDel collection: a set of P-element insertions for generating custom chromosomal aberrations in Drosophila melanogaster. Genetics, 167(2):797-813.
We describe a collection of P-element insertions that have considerable utility for generating custom chromosomal aberrations in Drosophila melanogaster. We have mobilized a pair of engineered P elements, p[RS3] and p[RS5], to collect 3243 lines unambiguously mapped to the Drosophila genome sequence. The collection contains, on average, an element every 35 kb. We demonstrate the utility of the collection for generating custom chromosomal deletions that have their end points mapped, with base-pair resolution, to the genome sequence. The collection was generated in an isogenic strain, thus affording a uniform background for screens where sensitivity to genetic background is high. The entire collection, along with a computational and genetic toolbox for designing and generating custom deletions, is publicly available. Using the collection it is theoretically possible to generate >12,000 deletions between 1 bp and 1 Mb in size by simple eye color selection. In addition, a further 37,000 deletions, selectable by molecular screening, may be generated. We are now using the collection to generate a second-generation deficiency kit that is precisely mapped to the genome sequence.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||07 Faculty of Science > Institute of Zoology (former)|
|DDC:||570 Life sciences; biology|
590 Animals (Zoology)
|Date:||01 June 2004|
|Deposited On:||11 Feb 2008 13:16|
|Last Modified:||28 Nov 2013 00:43|
|Publisher:||Genetics Society of America|
|Citations:||Web of Science®. Times cited: 147|
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