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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-58970

Blanc, F; Vissers, Y M; Adel-Patient, Karine; Rigby, N M; Mackie, A R; Gunning, A P; Wellner, N K; Skov, P S; Przybylski-Nicaise, L; Ballmer-Weber, B; Zuidmeer-Jongejan, L; Szépfalusi, Z; Ruinemans-Koerts, J; Jansen, A P H; Bernard, H; Wal, J M; Savelkoul, H F J; Wichers, H J; Mills, E N C (2011). Boiling peanut Ara h 1 results in the formation of aggregates with reduced allergenicity. Molecular Nutrition & Food Research, 55(12):1887-1894.

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Abstract

SCOPE:

Roasting rather than boiling and Maillard modifications may modulate peanut allergenicity. We investigated how these factors affect the allergenic properties of a major peanut allergen, Ara h 1.
METHODS AND RESULTS:

Ara h 1 was purified from either raw (N-Ara h 1) or roasted (R-Ara h 1) peanuts. Boiling (100°C 15 min; H-Ara h 1) resulted in a partial loss of Ara h 1 secondary structure and formation of rod-like branched aggregates with reduced IgE-binding capacity and impaired ability to induce mediator release. Glycated Ara h 1 (G-Ara h 1) formed by boiling in the presence of glucose behaved similarly. However, H- and G-Ara h1 retained the T-cell reactivity of N-Ara h 1. R-Ara h 1 was denatured, comprised compact, globular aggregates, and showed no evidence of glycation but retained the IgE-binding capacity of the native protein.
CONCLUSION:

Ara h 1 aggregates formed by boiling were morphologically distinct from those formed by roasting and had lower allergenic activity. Glycation had no additional effect on Ara h 1 allergenicity compared with heating alone. Taken together with published data on the loss of Ara h 2/6 from boiled peanuts, this supports the hypothesis that boiling reduces the allergenicity of peanuts.

Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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15 citations in Web of Science®
15 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Dermatology Clinic
DDC:610 Medicine & health
Language:English
Date:2011
Deposited On:26 Feb 2012 15:57
Last Modified:13 Jan 2014 13:46
Publisher:Wiley-VCH
ISSN:1613-4133
Publisher DOI:10.1002/mnfr.201100251
PubMed ID:22086730

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