UZH-Logo

Maintenance Infos

An international collaboration to standardize HIV-2 viral load assays: results from the 2009 ACHI(E)V(2E) quality control study


Damond, F; Benard, A; Balotta, C; Böni, J; Cotten, M; Duque, V; Ferns, B; Garson, J; Gomes, P; Gonçalves, F; Gottlieb, G; Kupfer, B; Ruelle, J; Rodes, B; Soriano, V; Wainberg, M; Taieb, A; Matheron, S; Chene, G; Brun-Vezinet, F (2011). An international collaboration to standardize HIV-2 viral load assays: results from the 2009 ACHI(E)V(2E) quality control study. Journal of Clinical Microbiology, 49(10):3491-3497.

Abstract

Accurate HIV-2 plasma viral load quantification is crucial for adequate HIV-2 patient management and for
the proper conduct of clinical trials and international cohort collaborations. This study compared the homogeneity
of HIV-2 RNA quantification when using HIV-2 assays from ACHIEV2E study sites and either in-house
PCR calibration standards or common viral load standards supplied to all collaborators. Each of the 12
participating laboratories quantified blinded HIV-2 samples, using its own HIV-2 viral load assay and standard
as well as centrally validated and distributed common HIV-2 group A and B standards (http://www.hiv
.lanl.gov/content/sequence/HelpDocs/subtypes-more.html). Aliquots of HIV-2 group A and B strains, each at 2
theoretical concentrations (2.7 and 3.7 log10 copies/ml), were tested. Intralaboratory, interlaboratory, and
overall variances of quantification results obtained with both standards were compared using F tests. For
HIV-2 group A quantifications, overall and interlaboratory and/or intralaboratory variances were significantly
lower when using the common standard than when using in-house standards at the concentration levels of 2.7
log10 copies/ml and 3.7 log10 copies/ml, respectively. For HIV-2 group B, a high heterogeneity was observed and
the variances did not differ according to the type of standard used. In this international collaboration, the use
of a common standard improved the homogeneity of HIV-2 group A RNA quantification only. The diversity of
HIV-2 group B, particularly in PCR primer-binding regions, may explain the heterogeneity in quantification
of this strain. Development of a validated HIV-2 viral load assay that accurately quantifies distinct circulating
strains is needed.

Abstract

Accurate HIV-2 plasma viral load quantification is crucial for adequate HIV-2 patient management and for
the proper conduct of clinical trials and international cohort collaborations. This study compared the homogeneity
of HIV-2 RNA quantification when using HIV-2 assays from ACHIEV2E study sites and either in-house
PCR calibration standards or common viral load standards supplied to all collaborators. Each of the 12
participating laboratories quantified blinded HIV-2 samples, using its own HIV-2 viral load assay and standard
as well as centrally validated and distributed common HIV-2 group A and B standards (http://www.hiv
.lanl.gov/content/sequence/HelpDocs/subtypes-more.html). Aliquots of HIV-2 group A and B strains, each at 2
theoretical concentrations (2.7 and 3.7 log10 copies/ml), were tested. Intralaboratory, interlaboratory, and
overall variances of quantification results obtained with both standards were compared using F tests. For
HIV-2 group A quantifications, overall and interlaboratory and/or intralaboratory variances were significantly
lower when using the common standard than when using in-house standards at the concentration levels of 2.7
log10 copies/ml and 3.7 log10 copies/ml, respectively. For HIV-2 group B, a high heterogeneity was observed and
the variances did not differ according to the type of standard used. In this international collaboration, the use
of a common standard improved the homogeneity of HIV-2 group A RNA quantification only. The diversity of
HIV-2 group B, particularly in PCR primer-binding regions, may explain the heterogeneity in quantification
of this strain. Development of a validated HIV-2 viral load assay that accurately quantifies distinct circulating
strains is needed.

Citations

11 citations in Web of Science®
12 citations in Scopus®
Google Scholar™

Altmetrics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Virology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:October 2011
Deposited On:05 Mar 2012 14:17
Last Modified:05 Apr 2016 15:38
Publisher:American Society for Microbiology (ASM)
ISSN:0095-1137
Publisher DOI:https://doi.org/10.1128/JCM.02389-10
PubMed ID:21813718

Download

Full text not available from this repository.
View at publisher

TrendTerms

TrendTerms displays relevant terms of the abstract of this publication and related documents on a map. The terms and their relations were extracted from ZORA using word statistics. Their timelines are taken from ZORA as well. The bubble size of a term is proportional to the number of documents where the term occurs. Red, orange, yellow and green colors are used for terms that occur in the current document; red indicates high interlinkedness of a term with other terms, orange, yellow and green decreasing interlinkedness. Blue is used for terms that have a relation with the terms in this document, but occur in other documents.
You can navigate and zoom the map. Mouse-hovering a term displays its timeline, clicking it yields the associated documents.

Author Collaborations