Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-60140
Gutzwiller, A; Hess, H D; Adam, A; Guggisberg, D; Liesegang, A; Stoll, P (2011). Effects of a reduced calcium, phosphorus and protein intake and of benzoic acid on calcium and phosphorus metabolism of growing pigs. Animal Feed Science and Technology, 168(1-2):113-121.
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In the last few years, various PCR based methods have been developed that enable detection of Cronobacter spp. to the genus and species level. Moreover, several real-time PCR based systems for detection of Cronobacter spp. are available, however, comparative evaluation studies are not available. The current study represents a comparative evaluation of three commercial diagnostic systems, namely the BAX® System PCR Assay Enterobacter sakazakii (DuPont, Qualicon, Wilmington, USA), the Assurance GDS™ Enterobacter sakazakii (BioControl, Bellvue, USA) and the foodproof® Enterobacter sakazakii Detection Kit (Biotecon Diagnostics, Potsdam, Germany) for the rapid identification of Cronobacter spp. Twenty-one target and non-target strains were included in the study and results were compared for specificity and convenience in performance. A specificity of 100% was observed for two of the three real time PCR systems tested, namely the Assurance GDS™ Enterobacter sakazakii and the foodproof® Enterobacter sakazakii Detection Kit for pure cultures as well as artificially contaminated powdered infant formula (PIF) samples.
Copyright © 2011 Elsevier B.V. All rights reserved.
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|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||05 Vetsuisse Faculty > Institute of Animal Nutrition|
|Dewey Decimal Classification:||570 Life sciences; biology
|Deposited On:||17 Mar 2012 19:26|
|Last Modified:||19 Dec 2013 08:11|
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