Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive 

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-60140

Gutzwiller, A; Hess, H D; Adam, A; Guggisberg, D; Liesegang, A; Stoll, P (2011). Effects of a reduced calcium, phosphorus and protein intake and of benzoic acid on calcium and phosphorus metabolism of growing pigs. Animal Feed Science and Technology, 168(1-2):113-121.

[img]Published Version
PDF - Registered users only
235kB

Abstract

In the last few years, various PCR based methods have been developed that enable detection of Cronobacter spp. to the genus and species level. Moreover, several real-time PCR based systems for detection of Cronobacter spp. are available, however, comparative evaluation studies are not available. The current study represents a comparative evaluation of three commercial diagnostic systems, namely the BAX® System PCR Assay Enterobacter sakazakii (DuPont, Qualicon, Wilmington, USA), the Assurance GDS™ Enterobacter sakazakii (BioControl, Bellvue, USA) and the foodproof® Enterobacter sakazakii Detection Kit (Biotecon Diagnostics, Potsdam, Germany) for the rapid identification of Cronobacter spp. Twenty-one target and non-target strains were included in the study and results were compared for specificity and convenience in performance. A specificity of 100% was observed for two of the three real time PCR systems tested, namely the Assurance GDS™ Enterobacter sakazakii and the foodproof® Enterobacter sakazakii Detection Kit for pure cultures as well as artificially contaminated powdered infant formula (PIF) samples.

Copyright © 2011 Elsevier B.V. All rights reserved.

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Animal Nutrition
DDC:570 Life sciences; biology
630 Agriculture
Language:English
Date:2011
Deposited On:17 Mar 2012 19:26
Last Modified:19 Dec 2013 08:11
Publisher:Elsevier
ISSN:0377-8401
Publisher DOI:10.1016/j.anifeedsci.2011.03.015
Citations:Web of Science®. Times Cited: 3
Google Scholar™
Scopus®. Citation Count: 3

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page