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Methicillin-resistant Staphylococcus lentus strains isolated from chicken carcasses and employees of a poultry abattoir


Koller, S; Huber, H; Cernela, N; Stephan, R; Zweifel, C (2011). Methicillin-resistant Staphylococcus lentus strains isolated from chicken carcasses and employees of a poultry abattoir. Journal of Food Safety and Food Quality, 62(4):136-140.

Abstract

Methicillin-resistant coagulase-negative staphylococci (MR-CNS) are increasingly reported in animals and humans as colonizing organisms and as opportunistic pathogens. Within a collection of MR-CNS isolates from livestock, chicken carcasses, bulk tank milk, minced meat, and contact persons, matrix-assisted laser desorption ionization-time of flight mass spectrometry identified 37 isolates as S. lentus. All 37 methicillin-resistant S. lentus strains originated from either chicken carcasses (30 strains) or employees working in a poultry abattoir (seven strains). To assess the phenotypic antibiotic resistance to selected antibiotics (ciprofloxacin, clindamycin, erythromycin, gentamicin, rifampin, sulfamethoxazole/trimethoprime, tetracycline, vancomycin), the disk diffusion method was used and none of the strains was resistant to gentamicin, rifampin, or vancomycin. Pulsed-field gel electrophoresis (SmaI) was applied to evaluate the genotypic relationship of the 37 methicillin-resistant S. lentus strains. With a cut-off level of 80 % similarity, 30 (81.1 %) strains were grouped into only two clusters. Overall, the seven human strains showed between 85 % and 100 % similarity to the closest related chicken isolates. Our results suggest potential transmission of methicillin-resistant S. lentus between slaughtered chickens and abattoir personnel.

Abstract

Methicillin-resistant coagulase-negative staphylococci (MR-CNS) are increasingly reported in animals and humans as colonizing organisms and as opportunistic pathogens. Within a collection of MR-CNS isolates from livestock, chicken carcasses, bulk tank milk, minced meat, and contact persons, matrix-assisted laser desorption ionization-time of flight mass spectrometry identified 37 isolates as S. lentus. All 37 methicillin-resistant S. lentus strains originated from either chicken carcasses (30 strains) or employees working in a poultry abattoir (seven strains). To assess the phenotypic antibiotic resistance to selected antibiotics (ciprofloxacin, clindamycin, erythromycin, gentamicin, rifampin, sulfamethoxazole/trimethoprime, tetracycline, vancomycin), the disk diffusion method was used and none of the strains was resistant to gentamicin, rifampin, or vancomycin. Pulsed-field gel electrophoresis (SmaI) was applied to evaluate the genotypic relationship of the 37 methicillin-resistant S. lentus strains. With a cut-off level of 80 % similarity, 30 (81.1 %) strains were grouped into only two clusters. Overall, the seven human strains showed between 85 % and 100 % similarity to the closest related chicken isolates. Our results suggest potential transmission of methicillin-resistant S. lentus between slaughtered chickens and abattoir personnel.

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Additional indexing

Other titles:Methicillin-resistente Staphylococcus lentus-Stämme isoliert von Geflügelschlachttierkörpern und Mitarbeitern eines Geflügelschlachthofs
Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2011
Deposited On:18 Mar 2012 13:13
Last Modified:05 Apr 2016 15:42
Publisher:Presse Dienstleistungsgesellschaft
ISSN:0003-925X
Additional Information:Zeitschrift früher u.d.T.: Archiv für Lebensmittelhygiene
Publisher DOI:https://doi.org/10.2376/0003-925X-62-136
Related URLs:http://opac.nebis.ch/F/?local_base=NEBIS&CON_LNG=GER&func=find-b&find_code=SYS&request=007165951
http://www.zora.uzh.ch/75512/

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