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The lmo0501 gene coding for a putative transcription activator protein in Listeria monocytogenes promotes growth under cold, osmotic and acid stress conditions


Michel, E. The lmo0501 gene coding for a putative transcription activator protein in Listeria monocytogenes promotes growth under cold, osmotic and acid stress conditions. 2011, University of Zurich, Vetsuisse Faculty.

Abstract

In L. monocytogenes EGDe, the lmo0501 gene locus encodes a protein similar to the mannitol
transcription regulator (MltR) protein in B. subtilis and B. stearothermophilus. In this study
we investigated its functional role in L. monocytogenes EGDe cells in view of growth under
different stress conditions. Increased lmo0501 gene expression at mRNA level was detected in
response to cold, osmotic and organic acid stress exposure. An EGDe Δlmo0501 mutant strain
was diminished in growth compared to the wild type strain in minimal defined medium
containing either glucose or fructose, as carbon sources. Furthermore the lmo0501 null mutant
had retarded growth under cold (4°C), salt (NaCl) and organic acid stress conditions
compared to the parental wild type strain. Our results confirm the role of the lmo0501 gene in
view of adaptation of L. monocytogenes cells to stress conditions as well as a contribution to
the efficient utilization of glucose and fructose as carbon sources.

In L. monocytogenes EGDe, the lmo0501 gene locus encodes a protein similar to the mannitol
transcription regulator (MltR) protein in B. subtilis and B. stearothermophilus. In this study
we investigated its functional role in L. monocytogenes EGDe cells in view of growth under
different stress conditions. Increased lmo0501 gene expression at mRNA level was detected in
response to cold, osmotic and organic acid stress exposure. An EGDe Δlmo0501 mutant strain
was diminished in growth compared to the wild type strain in minimal defined medium
containing either glucose or fructose, as carbon sources. Furthermore the lmo0501 null mutant
had retarded growth under cold (4°C), salt (NaCl) and organic acid stress conditions
compared to the parental wild type strain. Our results confirm the role of the lmo0501 gene in
view of adaptation of L. monocytogenes cells to stress conditions as well as a contribution to
the efficient utilization of glucose and fructose as carbon sources.

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Additional indexing

Item Type:Dissertation
Referees:Stephan R, Lindström M
Communities & Collections:05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2011
Deposited On:15 Mar 2012 21:20
Last Modified:05 Apr 2016 15:42
Additional Information:Vorzeitig publiziert in und Sonderdruck aus: Food Microbiology, 28(7):1261-1265. (s. ZORA: http://www.zora.uzh.ch/60453/)
Related URLs:http://opac.nebis.ch/F/?local_base=NEBIS&CON_LNG=GER&func=find-b&find_code=SYS&request=006577202
http://www.zora.uzh.ch/60453/
Permanent URL: https://doi.org/10.5167/uzh-60467

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