Haas, C; Hanson, E; Ballantyne, J (2012). Capillary electrophoresis of a multiplex reverse transcription-polymerase chain reaction to target messenger RNA markers for body fluid identification. In: Alonso, A. DNA Electrophoresis Protocols for Forensic Genetics. New York , 169-183. ISBN 978-1-61779-460-5 (P) 978-1-61779-461-2 (E).
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The analysis of cell-specific mRNA expression is a promising new method for the identification of body fluids. A number of mRNA markers have been identified for the forensically most relevant body fluids: blood, saliva, semen, vaginal secretions, and menstrual blood. Apart from a significant improvement in specificity compared to conventional protein-based methods, other important advantages of body fluid identification by mRNA profiling include the possibility of simultaneously isolating RNA and DNA from the same piece of stain and the ability to multiplex numerous RNA markers for the identification of one or several body fluids. RNA profiling can be incorporated into current DNA analysis pipelines.
|Item Type:||Book Section, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > Institute of Legal Medicine|
610 Medicine & health
|Deposited On:||17 Mar 2012 22:42|
|Last Modified:||28 Nov 2013 00:57|
|Series Name:||Methods in Molecular Biology|
|Number:||830, Pt. 1|
|ISBN:||978-1-61779-460-5 (P) 978-1-61779-461-2 (E)|
|Citations:||Web of Science®. Times Cited: 6|
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