The European Network of Forensic Science Institutes (ENFSI) and the European DNA Profiling Group (EDNAP) recommended the extension of the European Standard Set by 5 additional loci regarding international DNA data exchange based on the treaty of Prüm. This recommendation launched the development of new STR kits by-amongst others-Promega and Applied Biosystems (AB). Despite not being part of the EU nor the treaty of Prüm, Switzerland has decided to implement the new STR kits (including SE33). We therefore decided to conduct an in-house concordance study with 1498 reference samples analyzed with AmpFlSTR® SEfiler Plus™ and AmpFlSTR® NGM SElect™ (AB) and PowerPlex® ESX 17 and PowerPlex® ESI 17 (Promega). The concordance rate of the samples between the STR kits was greater than 99.967%.