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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-60707

Scharl, Michael; Frei, Sandra; Pesch, Theresa; Kellermeier, Silvia; Arikkat, Joba; Frei, Pascal; Fried, Michael; Weber, Achim; Jehle, Ekkehard; Rühl, Anne; Rogler, Gerhard (2013). Interleukin-13 and transforming growth factor β synergise in the pathogenesis of human intestinal fistulae. Gut, 62(1):63-72.

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Abstract

ObjectiveEpithelial to mesenchymal transition (EMT) seems to play an important role in the pathogenesis of fistulae, a common clinical complication of Crohn's disease (CD). TGFβ and interleukin-13 (IL-13) have been correlated with the onset of EMT-associated organ fibrosis and high levels of TGFβ have been shown in transitional cells (TCs) lining CD fistula tracts. This study investigated whether IL-13 could be involved in the pathogenesis of CD-associated fistulae.DesignProtein or mRNA levels in HT29 intestinal epithelial cells (IECs) or colonic lamina propria fibroblasts (CLPFs) were studied by western blotting or real-time PCR. CLPFs were isolated from non-inflammatory disease controls or patients with CD with or without fistulae and IL-13 levels were analysed in surgically removed fistula specimens by immunohistochemistry.ResultsTGFβ induced IL-13 secretion in CLPFs from patients with fistulising CD. In fistula specimens high levels of IL-13 were detected in TCs covering fistula tracts. In HT29 IEC monolayers, IL-13 induced SLUG and β6-integrin mRNA, which are associated with cell invasion. HT29 spheroids completely disintegrated when treated with TGFβ for 7 days, whereas IL-13-treated spheroids did not show morphological changes. Here, TGFβ induced mRNA expression of SNAIL1 and IL-13, whereas IL-13 elevated SLUG and β6-integrin mRNA. An anti-IL-13 antibody was able to prevent IL-13-induced SLUG expression in HT29 IECs.ConclusionsTGFβ induces IL-13 expression and an EMT-like phenotype of IECs, while IL-13 promotes the expression of genes associated with cell invasion. These findings suggest that TGFβ and IL-13 play a synergistic role in the pathogenesis of fistulae and inhibition of IL-13 might represent a novel therapeutic approach for fistula treatment.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Surgical Pathology
04 Faculty of Medicine > University Hospital Zurich > Clinic for Gastroenterology and Hepatology
DDC:610 Medicine & health
Language:English
Date:2013
Deposited On:10 Apr 2012 09:32
Last Modified:10 Dec 2013 06:11
Publisher:BMJ Publishing Group
ISSN:0017-5749
Publisher DOI:10.1136/gutjnl-2011-300498
PubMed ID:22287592
Citations:Web of Science®. Times Cited: 7
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