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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-61843

Haenni, Simon; Ji, Zhe; Hoque, Mainul; Rust, Nigel; Sharpe, Helen; Eberhard, Ralf; Browne, Cathy; Hengartner, Michael O; Mellor, Jane; Tian, Bin; Furger, André (2012). Analysis of C. elegans intestinal gene expression and polyadenylation by fluorescence-activated nuclei sorting and 3'-end-seq. Nucleic Acids Research, 40(13):6304-6318.

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Abstract

Despite the many advantages of Caenorhabditis elegans, biochemical approaches to study tissue-specific gene expression in post-embryonic stages are challenging. Here, we report a novel experimental approach for efficient determination of tissue-specific transcriptomes involving the rapid release and purification of nuclei from major tissues of post-embryonic animals by fluorescence-activated nuclei sorting (FANS), followed by deep sequencing of linearly amplified 3'-end regions of transcripts (3'-end-seq). We employed these approaches to compile the transcriptome of the developed C. elegans intestine and used this to analyse tissue-specific cleavage and polyadenylation. In agreement with intestinal-specific gene expression, highly expressed genes have enriched GATA-elements in their promoter regions and their functional properties are associated with processes that are characteristic for the intestine. We systematically mapped pre-mRNA cleavage and polyadenylation sites, or polyA sites, including more than 3000 sites that have previously not been identified. The detailed analysis of the 3'-ends of the nuclear mRNA revealed widespread alternative polyA site use (APA) in intestinally expressed genes. Importantly, we found that intestinal polyA sites that undergo APA tend to have U-rich and/or A-rich upstream auxiliary elements that may contribute to the regulation of 3'-end formation in the intestine.

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
DDC:570 Life sciences; biology
Language:English
Date:29 March 2012
Deposited On:26 Apr 2012 08:50
Last Modified:28 Nov 2013 04:37
Publisher:Oxford University Press
ISSN:0305-1048
Publisher DOI:10.1093/nar/gks282
PubMed ID:22467213
Citations:Web of Science®. Times Cited: 6
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