Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-61882
Arras, Margarete; Glauser, Daniel L; Jirkof, Paulin; Rettich, Andreas; Schade, Benjamin; Cinelli, Paolo; Pinschewer, Daniel D; Ackermann, Mathias (2012). Multiparameter telemetry as a sensitive screening method to detect vaccine reactogenicity in mice. PLoS ONE, 7(1):e29726.
|Published Version (English)|
Refined vaccines and adjuvants are urgently needed to advance immunization against global infectious challenges such as HIV, hepatitis C, tuberculosis and malaria. Large-scale screening efforts are ongoing to identify adjuvants with improved efficacy profiles. Reactogenicity often represents a major hurdle to the clinical use of new substances. Yet, irrespective of its importance, this parameter has remained difficult to screen for, owing to a lack of sensitive small animal models with a capacity for high throughput testing. Here we report that continuous telemetric measurements of heart rate, heart rate variability, body core temperature and locomotor activity in laboratory mice readily unmasked systemic side-effects of vaccination, which went undetected by conventional observational assessment and clinical scoring. Even minor aberrations in homeostasis were readily detected, ranging from sympathetic activation over transient pyrogenic effects to reduced physical activity and apathy. Results in real-time combined with the potential of scalability and partial automation in the industrial context suggest multiparameter telemetry in laboratory mice as a first-line screen for vaccine reactogenicity. This may accelerate vaccine discovery in general and may further the success of vaccines in combating infectious disease and cancer.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||05 Vetsuisse Faculty > Institute of Veterinary Pathology|
05 Vetsuisse Faculty > Institute of Laboratory Animal Science
04 Faculty of Medicine > Institute of Laboratory Animal Science
05 Vetsuisse Faculty > Institute of Virology
|DDC:||570 Life sciences; biology|
610 Medicine & health
|Deposited On:||27 Apr 2012 08:38|
|Last Modified:||30 Nov 2012 18:44|
|Publisher:||Public Library of Science|
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