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Analysis of Swiss swine influenza virus genomes


Heidemeyer, Silke Johanna Alexia. Analysis of Swiss swine influenza virus genomes. 2012, University of Zurich, Vetsuisse Faculty.

Abstract

The aim of this work was to complete for the first time a full set of Swiss Swine Influenza Virus (SSIV) genome sequences. A previously published method (Zhou et al., 2009, J. Virol. 83, 10309-10313) for multi-segment amplification of influenza genomes was used in combination with a panel of 49 SSIV isolates collected between 2004 and 2010. A total of 55 sequences were obtained, covering 15 isolates, 9 representing strains that had previously been propagated in cell culture and 6 derived directly from nasal swabs of pigs with influenza-like disease symptoms. Analyses of the sequences indicated that the SSIV strains were clearly distinct from the recent pandemic H1N1 strain (Mexico 2009) and were related more closely among each other than to a well-characterized European SIV (Haseluenne 2003). All isolates, of which the neuraminidase (NA) sequence could be determined, were Oseltamivir resistant (H274Y mutation in NA) and featured the R194G mutation in NA, a prerequisite for the consecutive H274Y mutation. Moreover, the predicted membrane protein 2 (M2) aa sequences suggested resistance against the drug Amantadine. Although the pandemic H1N1 circulated in Switzerland already in 2009, it was observed only in 2011 to entering the Swiss pig population, suggesting that this virus actually represents zoonotic features, though, in the case of Switzerland, not originating from pigs and transmitted to humans but rather contrary, originating in humans and transmitted to pigs.

The aim of this work was to complete for the first time a full set of Swiss Swine Influenza Virus (SSIV) genome sequences. A previously published method (Zhou et al., 2009, J. Virol. 83, 10309-10313) for multi-segment amplification of influenza genomes was used in combination with a panel of 49 SSIV isolates collected between 2004 and 2010. A total of 55 sequences were obtained, covering 15 isolates, 9 representing strains that had previously been propagated in cell culture and 6 derived directly from nasal swabs of pigs with influenza-like disease symptoms. Analyses of the sequences indicated that the SSIV strains were clearly distinct from the recent pandemic H1N1 strain (Mexico 2009) and were related more closely among each other than to a well-characterized European SIV (Haseluenne 2003). All isolates, of which the neuraminidase (NA) sequence could be determined, were Oseltamivir resistant (H274Y mutation in NA) and featured the R194G mutation in NA, a prerequisite for the consecutive H274Y mutation. Moreover, the predicted membrane protein 2 (M2) aa sequences suggested resistance against the drug Amantadine. Although the pandemic H1N1 circulated in Switzerland already in 2009, it was observed only in 2011 to entering the Swiss pig population, suggesting that this virus actually represents zoonotic features, though, in the case of Switzerland, not originating from pigs and transmitted to humans but rather contrary, originating in humans and transmitted to pigs.

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Additional indexing

Item Type:Dissertation
Referees:Ackermann M, Bertoni Giuseppe
Communities & Collections:05 Vetsuisse Faculty > Institute of Virology
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:2012
Deposited On:14 Aug 2012 12:57
Last Modified:05 Apr 2016 15:55
Related URLs:http://opac.nebis.ch/F/?local_base=NEBIS&CON_LNG=GER&func=find-b&find_code=SYS&request=007335074
Permanent URL: https://doi.org/10.5167/uzh-64003

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