Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-643
Nairz, K; Zipperlen, P; Dearolf, C; Basler, K; Hafen, E (2004). A reverse genetic screen in Drosophila using a deletion-inducing mutagen. Genome Biology, 5(10):R83.
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We report the use of the cross-linking drug hexamethylphosphoramide (HMPA), which introduces small deletions, as a mutagen suitable for reverse genetics in the model organism Drosophila melanogaster. A compatible mutation-detection method based on resolution of PCR fragment-length polymorphisms on standard DNA sequencers is implemented. As the spectrum of HMPA-induced mutations is similar in a variety of organisms, it should be possible to transfer this mutagenesis and detection procedure to other model systems.
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|Item Type:||Journal Article, refereed|
|Communities & Collections:||07 Faculty of Science > Institute of Zoology (former)
07 Faculty of Science > Institute of Molecular Life Sciences
|DDC:||570 Life sciences; biology
590 Animals (Zoology)
|Deposited On:||11 Feb 2008 12:16|
|Last Modified:||28 Nov 2013 01:24|
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