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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-643

Nairz, K; Zipperlen, P; Dearolf, C; Basler, K; Hafen, E (2004). A reverse genetic screen in Drosophila using a deletion-inducing mutagen. Genome Biology, 5(10):R83.

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Abstract

We report the use of the cross-linking drug hexamethylphosphoramide (HMPA), which introduces small deletions, as a mutagen suitable for reverse genetics in the model organism Drosophila melanogaster. A compatible mutation-detection method based on resolution of PCR fragment-length polymorphisms on standard DNA sequencers is implemented. As the spectrum of HMPA-induced mutations is similar in a variety of organisms, it should be possible to transfer this mutagenesis and detection procedure to other model systems.

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3 citations in Web of Science®
2 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed
Communities & Collections:07 Faculty of Science > Institute of Zoology (former)
07 Faculty of Science > Institute of Molecular Life Sciences
DDC:570 Life sciences; biology
590 Animals (Zoology)
Language:English
Date:2004
Deposited On:11 Feb 2008 12:16
Last Modified:28 Nov 2013 01:24
Publisher:BioMed Central
ISSN:1465-6906
Publisher DOI:10.1186/gb-2004-5-10-r83
PubMed ID:15461801

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