Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-643
Nairz, K; Zipperlen, P; Dearolf, C; Basler, K; Hafen, E (2004). A reverse genetic screen in Drosophila using a deletion-inducing mutagen. Genome Biology, 5(10):R83.
View at publisher
We report the use of the cross-linking drug hexamethylphosphoramide (HMPA), which introduces small deletions, as a mutagen suitable for reverse genetics in the model organism Drosophila melanogaster. A compatible mutation-detection method based on resolution of PCR fragment-length polymorphisms on standard DNA sequencers is implemented. As the spectrum of HMPA-induced mutations is similar in a variety of organisms, it should be possible to transfer this mutagenesis and detection procedure to other model systems.
92 downloads since deposited on 11 Feb 2008
28 downloads since 12 months
|Item Type:||Journal Article, refereed|
|Communities & Collections:||07 Faculty of Science > Institute of Zoology (former)
07 Faculty of Science > Institute of Molecular Life Sciences
|Dewey Decimal Classification:||570 Life sciences; biology
590 Animals (Zoology)
|Deposited On:||11 Feb 2008 12:16|
|Last Modified:||28 Nov 2013 01:24|
Users (please log in): suggest update or correction for this item
Repository Staff Only: item control page