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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-64461

Mohebbi, Nilufar; Benabbas, Chahira; Vidal, Solange; Daryadel, Arezoo; Bourgeois, Soline; Velic, Ana; Ludwig, Marie-Gabrielle; Seuwen, Klaus; Wagner, Carsten A (2012). The proton-activated G protein coupled receptor OGR1 acutely regulates the activity of epithelial proton transport proteins. Cellular Physiology and Biochemistry, 29(3-4):313-324.

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Abstract

The Ovarian cancer G protein-coupled Receptor 1 (OGR1; GPR68) is proton-sensitive in the pH range of 6.8 - 7.8. However, its physiological function is not defined to date. OGR1 signals via inositol trisphosphate and intracellular calcium, albeit downstream events are unclear. To elucidate OGR1 function further, we transfected HEK293 cells with active OGR1 receptor or a mutant lacking 5 histidine residues (H5Phe-OGR1). An acute switch of extracellular pH from 8 to 7.1 (10 nmol/l vs 90 nmol/l protons) stimulated NHE and H(+)-ATPase activity in OGR1-transfected cells, but not in H5Phe-OGR1-transfected cells. ZnCl(2) and CuCl(2) that both inhibit OGR1 reduced the stimulatory effect. The activity was blocked by chelerythrine, whereas the ERK1/2 inhibitor PD 098059 had no inhibitory effect. OGR1 activation increased intracellular calcium in transfected HEK293 cells. We next isolated proximal tubules from kidneys of wild-type and OGR1-deficient mice and measured the effect of extracellular pH on NHE activity in vitro. Deletion of OGR1 affected the pH-dependent proton extrusion, however, in the opposite direction as expected from cell culture experiments. Upregulated expression of the pH-sensitive kinase Pyk2 in OGR1 KO mouse proximal tubule cells may compensate for the loss of OGR1. Thus, we present the first evidence that OGR1 modulates the activity of two major plasma membrane proton transport systems. OGR1 may be involved in the regulation of plasma membrane transport proteins and intra- and/or extracellular pH.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology

04 Faculty of Medicine > Center for Integrative Human Physiology
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2012
Deposited On:12 Sep 2012 08:10
Last Modified:03 Dec 2013 11:40
Publisher:Karger
ISSN:1015-8987
Additional Information:© 2012 S. Karger AG
Publisher DOI:10.1159/000338486
PubMed ID:22508039
Citations:Web of Science®. Times Cited: 8
Google Scholar™
Scopus®. Citation Count: 8

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