Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-64522
O'Connor, Tracy; Frei, Nathalie; Sponarova, Jana; Schwarz, Petra; Heikenwalder, Mathias; Aguzzi, Adriano (2012). Lymphotoxin, but Not TNF, Is Required for Prion Invasion of Lymph Nodes. PLoS Pathogens, 8(8):e1002867.
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Neuroinvasion and subsequent destruction of the central nervous system by prions are typically preceded by a colonization phase in lymphoid organs. An important compartment harboring prions in lymphoid tissue is the follicular dendritic cell (FDC), which requires both tumor necrosis factor receptor 1 (TNFR1) and lymphotoxin β receptor (LTβR) signaling for maintenance. However, prions are still detected in TNFR1(-/-) lymph nodes despite the absence of mature FDCs. Here we show that TNFR1-independent prion accumulation in lymph nodes depends on LTβR signaling. Loss of LTβR signaling, but not of TNFR1, was concurrent with the dedifferentiation of high endothelial venules (HEVs) required for lymphocyte entry into lymph nodes. Using luminescent conjugated polymers for histochemical PrP(Sc) detection, we identified PrP(Sc) deposits associated with HEVs in TNFR1(-/-) lymph nodes. Hence, prions may enter lymph nodes by HEVs and accumulate or replicate in the absence of mature FDCs.
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|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology|
|DDC:||570 Life sciences; biology
610 Medicine & health
|Deposited On:||03 Sep 2012 13:25|
|Last Modified:||26 Nov 2012 10:07|
|Publisher:||Public Library of Science|
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