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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-64675

Weiler, M; Pfenning, P N; Thiepold, A L; Blaes, J; Jestaedt, L; Gronych, J; Dittmann, L M; Berger, B; Jugold, M; Kosch, M; Combs, S E; von Deimling, A; Weller, M; Bendszus, M; Platten, M; Wick, W (2013). Suppression of proinvasive RGS4 by mTOR inhibition optimizes glioma treatment. Oncogene, 32(9):1099-1109.

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Abstract

An essential mode of acquired resistance to radiotherapy (RT) appears to be promotion of tumor cell motility and invasiveness in various cancer types, including glioblastoma, a process resembling 'evasive resistance'. Hence, a logical advancement of RT would be to identify suitable complementary treatment strategies, ideally targeting cell motility. Here we report that the combination of focal RT and mammalian target of rapamycin (mTOR) inhibition using clinically relevant concentrations of temsirolimus (CCI-779) prolongs survival in a syngeneic mouse glioma model through additive cytostatic effects. In vitro, the mTOR inhibitor CCI-779 exerted marked anti-invasive effects, irrespective of the phosphatase and tensin homolog deleted on chromosome 10 status and counteracted the proinvasive effect of sublethal irradiation. Mechanistically, we identified regulator of G-protein signaling 4 (RGS4) as a novel target of mTOR inhibition and a key driver of glioblastoma invasiveness, sensitive to the anti-invasive properties of CCI-779. Notably, suppression of RGS4-dependent glioma cell invasion was signaled through both mTOR complexes, mTORC1 and mTORC2, in a concentration-dependent manner, indicating that high doses of CCI-779 may overcome tumor-cell resistance associated with the sole inhibition of mTORC1. We conclude that combined RT and mTOR inhibition is a promising therapeutic option that warrants further clinical investigation in upfront glioblastoma therapy.Oncogene advance online publication, 7 May 2012; doi:10.1038/onc.2012.137.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Neurology
DDC:610 Medicine & health
Language:English
Date:2013
Deposited On:08 Oct 2012 13:02
Last Modified:27 Nov 2013 19:27
Publisher:Nature Publishing Group
ISSN:0950-9232
Publisher DOI:10.1038/onc.2012.137
PubMed ID:22562250
Citations:Web of Science®. Times Cited: 2
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Scopus®. Citation Count: 3

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