Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-64699

Hermann, Mario; Maeder, Morgan L; Rector, Kyle; Ruiz, Joseph; Becher, Burkhard; Bürki, Kurt; Khayter, Cyd; Aguzzi, Adriano; Joung, J Keith; Buch, Thorsten; Pelczar, Pawel (2012). Evaluation of OPEN Zinc finger nucleases for direct gene targeting of the ROSA26 locus in mouse embryos. PLoS ONE, 7(9):e41796.

[img]
Preview
Published Version
PDF
387kB

View at publisher

Abstract

Zinc finger nucleases (ZFNs) enable precise genome modification in a variety of organisms and cell types. Commercial ZFNs were reported to enhance gene targeting directly in mouse zygotes, whereas similar approaches using publicly available resources have not yet been described. Here we report precise targeted mutagenesis of the mouse genome using Oligomerized Pool Engineering (OPEN) ZFNs. OPEN ZFN can be constructed using publicly available resources and therefore provide an attractive alternative for academic researchers. Two ZFN pairs specific to the mouse genomic locus gt(ROSA26)Sor were generated by OPEN selections and used for gene disruption and homology-mediated gene replacement in single cell mouse embryos. One specific ZFN pair facilitated non-homologous end joining (NHEJ)-mediated gene disruption when expressed in mouse zygotes. We also observed a single homologous recombination (HR)-driven gene replacement event when this ZFN pair was co-injected with a targeting vector. Our experiments demonstrate the feasibility of achieving both gene ablation through NHEJ and gene replacement by HR by using the OPEN ZFN technology directly in mouse zygotes.

Citations

9 citations in Web of Science®
12 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

27 downloads since deposited on 19 Sep 2012
20 downloads since 12 months

Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology
05 Vetsuisse Faculty > Institute of Laboratory Animal Science
04 Faculty of Medicine > Institute of Laboratory Animal Science
DDC:570 Life sciences; biology
610 Medicine & health
Language:English
Date:September 2012
Deposited On:19 Sep 2012 08:52
Last Modified:12 Nov 2014 12:24
Publisher:Public Library of Science (PLoS)
ISSN:1932-6203
Publisher DOI:10.1371/journal.pone.0041796
PubMed ID:22970113

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page