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mRNA profiling for the identification of blood—Results of a collaborative EDNAP exercise


Haas, C; Hanson, E; Bär, W; Banemann, R; Bento, A M; Berti, A; Borges, E; Bouakaze, C; Carracedo, A; Carvalho, M; Choma, A; Dötsch, M; Durianciková, M; Hoff-Olsen, P; Hohoff, C; Johansen, P; Lindenbergh, P A; Loddenkötter, B; Ludes, B; Maroñas, O; Morling, N; Niederstätter, H; Parson, W; Patel, G; Popielarz, C; Salata, E; Schneider, P M; Sijen, T; Sviezená, B; Zatkalíková, L; Ballantyne, J (2011). mRNA profiling for the identification of blood—Results of a collaborative EDNAP exercise. Forensic Science International: Genetics, 5(1):21-26.

Abstract

A collaborative exercise on mRNA profiling for the identification of blood was organized by the European DNA Profiling Group (EDNAP). Seven blood samples and one blood dilution series were analyzed by the participating laboratories for the reportedly blood-specific markers HBB, SPTB and PBGD, using different kits, chemistries and instrumentation. The results demonstrate that HBB is expressed abundantly in blood, SPTB moderately and PBGD significantly less. All but one of the 16 participating laboratories were able to successfully isolate and detect RNA from the dried bloodstains even though a majority of the laboratories had no prior experience with RNA. Despite some expected variation in sensitivity between laboratories, the method proved to be reproducible and sensitive using different analysis strategies. The results of this collaborative exercise support the potential use of mRNA profiling as an alternative to conventional serological tests.

A collaborative exercise on mRNA profiling for the identification of blood was organized by the European DNA Profiling Group (EDNAP). Seven blood samples and one blood dilution series were analyzed by the participating laboratories for the reportedly blood-specific markers HBB, SPTB and PBGD, using different kits, chemistries and instrumentation. The results demonstrate that HBB is expressed abundantly in blood, SPTB moderately and PBGD significantly less. All but one of the 16 participating laboratories were able to successfully isolate and detect RNA from the dried bloodstains even though a majority of the laboratories had no prior experience with RNA. Despite some expected variation in sensitivity between laboratories, the method proved to be reproducible and sensitive using different analysis strategies. The results of this collaborative exercise support the potential use of mRNA profiling as an alternative to conventional serological tests.

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Additional indexing

Item Type:Journal Article, not refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Legal Medicine
Dewey Decimal Classification:340 Law
610 Medicine & health
Language:English
Date:2011
Deposited On:20 Sep 2012 12:19
Last Modified:05 Apr 2016 15:57
Publisher:Elsevier
ISSN:1872-4973
Publisher DOI:https://doi.org/10.1016/j.fsigen.2010.01.003
PubMed ID:20457073
Permanent URL: https://doi.org/10.5167/uzh-64730

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