Kratochvil, Milos; Rümmelein, Bettina; Reimers, Uta; Ehlert, Ulrike; Weichenthal, Michael; Mensing, Hartwig; Breitbart, Eckhard W; Rüdiger, Hugo W (1991). Constitutively increased micronuclei are predominantly caused by acentric fragments. Mutation Research, 249(1):223-226.
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The frequency of kinetochore (centromere)-positive micronuclei (MN) was determined in 32 fibroblast cell lines. We tested 16 probands with spontaneously high MN levels (greater than or equal to 20 MN/500 cells (4%] and 8 probands (controls) with low MN levels (less than or equal to 13 MN/500 cells (2.6%]. To study whether the elevation of MN levels is due to increased chromosomal breakage we used the antikinetochore antibody fluorescent staining method. Probands with spontaneously high MN had kinetochore-positive MN increased by a factor 2.1 compared to the controls whereas the kinetochore-negative MN were increased by a factor 6.14. This shows that spontaneous elevation of MN is mainly caused by increased chromosomal breakage and only in a minor proportion by chromosome segregation errors as a consequence of spindle defects.
|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||06 Faculty of Arts > Institute of Psychology|
|Deposited On:||23 Oct 2012 13:51|
|Last Modified:||27 Nov 2013 22:10|
|Citations:||Web of Science®. Times cited: 6|
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