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Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-66012

Sutter, Esther; de Oliveira, Anna Paula; Tobler, Kurt; Schraner, Elisabeth M.; Sonda, Sabrina; Kaech, Andres; Lucas, Miriam S; Ackermann, M; Wild, Peter (2012). Herpes simplex virus 1 induces de novo phospholipid synthesis. Virology, 429(2):124-135.

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Abstract

Herpes simplex virus type 1 capsids bud at nuclear membranes and Golgi membranes acquiring an envelope composed of phospholipids. Hence, we measured incorporation of phospholipid precursors into these membranes, and quantified changes in size of cellular compartments by morphometric analysis. Incorporation of [³H]-choline into both nuclear and cytoplasmic membranes was significantly enhanced upon infection. [³H]-choline was also part of isolated virions even grown in the presence of brefeldin A. Nuclei expanded early in infection. The Golgi complex and vacuoles increased substantially whereas the endoplasmic reticulum enlarged only temporarily. The data suggest that HSV-1 stimulates phospholipid synthesis, and that de novo synthesized phospholipids are inserted into nuclear and cytoplasmic membranes to i) maintain membrane integrity in the course of nuclear and cellular expansion, ii) to supply membrane constituents for envelopment of capsids by budding at nuclear membranes and Golgi membranes, and iii) to provide membranes for formation of transport vacuoles.

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Anatomy
05 Vetsuisse Faculty > Institute of Parasitology
04 Faculty of Medicine > Institute of Parasitology

04 Faculty of Medicine > University Hospital Zurich > Clinic for Visceral and Transplantation Surgery
05 Vetsuisse Faculty > Institute of Virology
DDC:570 Life sciences; biology
610 Medicine & health
600 Technology
Language:English
Date:May 2012
Deposited On:30 Oct 2012 17:17
Last Modified:01 Dec 2013 01:49
Publisher:Elsevier
ISSN:0042-6822
Publisher DOI:10.1016/j.virol.2012.04.004
PubMed ID:22560864
Citations:Web of Science®. Times Cited: 2
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