Quick Search:

uzh logo
Browse by:

Zurich Open Repository and Archive

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-66014

Wild, Peter; de Oliveira, Anna Paula; Sonda, Sabrina; Schraner, Elisabeth M; Ackermann, Mathias; Tobler, Kurt (2012). The herpes simplex virus 1 U(S)3 regulates phospholipid synthesis. Virology, 432(2):353-360.

[img] PDF - Registered users only
View at publisher


Herpes simplex virus type 1 capsids bud at nuclear and Golgi membranes for envelopment by phospholipid bilayers. In the absence of U(S)3, nuclear membranes form multiple folds harboring virions that suggests disturbance in membrane turnover. Therefore, we investigated phospholipid metabolism in cells infected with the U(S)3 deletion mutant R7041(ΔU(S)3), and quantified membranes involved in viral envelopment. We report that (i) [(3)H]-choline incorporation into nuclear membranes and cytoplasmic membranes was enhanced peaking at 12 or 20 h post inoculation with wild type HSV-1 and R7041(ΔU(S)3), respectively, (ii) the surface area of nuclear membranes increased until 24 h of R7041(ΔU(S)3) infection forming folds that equaled ~45% of the nuclear surface, (iii) the surface area of viral envelopes between nuclear membranes equaled ~2400 R7041(ΔU(S)3) virions per cell, and (iv) during R7041(ΔU(S)3) infection, the Golgi complex expanded dramatically. The data indicate that U(S)3 plays a significant role in regulation of membrane biosynthesis.


3 citations in Web of Science®
4 citations in Scopus®
Google Scholar™



3 downloads since deposited on 30 Oct 2012
0 downloads since 12 months

Detailed statistics

Additional indexing

Other titles:The herpes simplex virus 1 US3 regulates phospholipid synthesis
Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Microscopy and Image Analysis
05 Vetsuisse Faculty > Institute of Veterinary Anatomy
05 Vetsuisse Faculty > Institute of Parasitology
04 Faculty of Medicine > Institute of Parasitology

04 Faculty of Medicine > University Hospital Zurich > Clinic for Visceral and Transplantation Surgery
05 Vetsuisse Faculty > Institute of Virology
Dewey Decimal Classification:570 Life sciences; biology
600 Technology
610 Medicine & health
Date:July 2012
Deposited On:30 Oct 2012 16:12
Last Modified:05 Apr 2016 16:02
Publisher DOI:10.1016/j.virol.2012.06.020
PubMed ID:22789738

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page