Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-6638
Todesco, L; Bur, D; Brooks, H; Török, M; Landmann, L; Stieger, B; Krähenbühl, S (2008). Pharmacological manipulation of L-carnitine transport into L6 cells with stable overexpression of human OCTN2. Cellular and Molecular Life Sciences, 65(10):1596-1608.
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The high-affinity Na+-dependent carnitine transporter OCTN2 (SLC22A5) has a high renal expression and reabsorbs most filtered carnitine. To gain more insight into substrate specificity of OCTN2, we overexpressed hOCTN2 in L6 cells and characterized the structural requirements of substances acting as human OCTN2 (hOCTN2) inhibitors. A 1905-bp fragment containing the hOCTN2 complete coding sequence was introduced into the pWpiresGFP vector, and L6 cells were stably transduced using a lentiviral system. The transduced L6 cells revealed increased expression of hOCTN2 on the mRNA, protein and functional levels. Structural requirements for hOCTN2 inhibition were predicted in silico and investigated in vitro. Essential structural requirements for OCTN2 inhibition include a constantly positively charged nitrogen atom and a carboxyl, nitrile or ester group connected by a 2-4-atom linker. Our cell system is suitable for studying in vitro interactions with OCTN2, which can subsequently be investigated in vivo.
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|Item Type:||Journal Article, refereed, original work|
|Communities & Collections:||04 Faculty of Medicine > University Hospital Zurich > Clinic for Clinical Pharmacology and Toxicology|
|Dewey Decimal Classification:||610 Medicine & health|
|Deposited On:||10 Dec 2008 08:00|
|Last Modified:||28 Nov 2013 01:16|
|Additional Information:||The original publication is available at www.springerlink.com|
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