Quick Search:

uzh logo
Browse by:
bullet
bullet
bullet
bullet

Zurich Open Repository and Archive 

Permanent URL to this publication: http://dx.doi.org/10.5167/uzh-6807

Guiducci, S; Distler, J H W; Jüngel, A; Huscher, D; Huber, L C; Michel, B A; Gay, R E; Pisetsky, D S; Gay, S; Matucci-Cerinic, M; Distler, O (2008). The relationship between plasma microparticles and disease manifestations in patients with systemic sclerosis. Arthritis and Rheumatism, 58(9):2845-2853.

[img]
Preview
Accepted Version
PDF
1MB
[img] PDF - Registered users only
1MB

Abstract

OBJECTIVE: Microparticles are small, membrane-coated vesicles that can serve as novel signaling structures between cells. The aim of the present study was to analyze the profile of microparticles in the blood of patients with systemic sclerosis (SSc; scleroderma) and healthy controls. METHODS: The study population consisted of 37 patients with SSc and 15 healthy subjects of comparable sex and age. Microparticles were isolated from plasma by high-speed differential centrifugation. Microparticles were stained with monoclonal antibodies against cell type-specific markers and were quantified by fluorescence-activated cell sorting analyses. RESULTS: The total number of microparticles was strongly increased in patients with SSc compared with healthy controls (mean +/- SEM 88.0 +/- 4.8 x 10(5) microparticles/ml plasma versus 42.3 +/- 9.4 x 10(5) microparticles/ml plasma; P < 0.001). Similarly, significant increases were found for microparticles derived from platelets, endothelial cells, monocytes, and T cells, reflecting the activation of these cells in SSc. Platelets were the most common source of microparticles in the blood of patients with SSc (66.9 +/- 5.2% of all microparticles) and healthy donors, followed by microparticles derived from endothelial cells (8.8 +/- 0.9% in SSc patients). The modified Rodnan skin thickness score (MRSS) was inversely correlated with the total number of microparticles. Furthermore, patients with cutaneous ulcers showed a significantly lower total number of microparticles. In multivariate analysis, an additive model of age, C-reactive protein, MRSS, and subtype of disease accounted for 55% of the variability of the total microparticle count (r = 0.744). CONCLUSION: The number of microparticles from different cellular sources is increased in the blood of SSc patients. Considering their role as important mediators of intercellular communication, microparticles could be a novel link between activated cellular compartments in the pathogenesis of SSc.

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Rheumatology Clinic and Institute of Physical Medicine
DDC:610 Medicine & health
Language:English
Date:2008
Deposited On:04 Dec 2008 07:44
Last Modified:28 Nov 2013 01:21
Publisher:Wiley-Blackwell
ISSN:0004-3591
Additional Information:The attached file is a preprint (accepted version) of an article published in: Arthritis and Rheumatism,2008;58(9).
Publisher DOI:10.1002/art.23735
PubMed ID:18759303
Citations:Web of Science®. Times Cited: 32
Google Scholar™
Scopus®. Citation Count: 32

Users (please log in): suggest update or correction for this item

Repository Staff Only: item control page